Glucocerebrosidase mutations in a mouse synucleinopathy model
小鼠突触核蛋白病模型中的葡萄糖脑苷脂酶突变
基本信息
- 批准号:7777864
- 负责人:
- 金额:$ 13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-03-01 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:AccountingAffectAllelesAlzheimer&aposs DiseaseAnimalsAnterior Horn CellsArtificial ChromosomesAuditoryAutonomic DysfunctionAutonomic nervous systemAutopsyBrainBrain DiseasesBrain regionBreedingCatecholaminesCharacteristicsCorpus striatum structureCultured CellsCytoplasmic ProteinDefectDementiaDiffuseDiseaseDisease ProgressionDisease susceptibilityElderlyEnzymesEtiologyFamilyFrequenciesFunctional disorderGastrointestinal MotilityGaucher DiseaseGene MutationGene ProteinsGenesGeneticGenetic ResearchGoalsHalf-LifeHumanHuman GeneticsInfectionInjection of therapeutic agentInterventionKnockout MiceLewy BodiesLewy Body DiseaseLysosomal Storage DiseasesMental DepressionMissense MutationModelingMotorMovement DisordersMusMutationNerve DegenerationNervous System PartNeuraxisNeurodegenerative DisordersNeuronsOdds RatioOocytesParkinson DiseaseParkinsonian DisordersPatientsPatternPhasePhenotypePhosphoproteinsPredispositionProcessProteinsPsychotic DisordersRNA InterferenceReactive Oxygen SpeciesRegulatory ElementResearchRisk FactorsRodent ModelRotenoneSNCA geneSigns and SymptomsStressSubfamily lentivirinaeSubstantia nigra structureTechnologyTestingTransgenesTransgenic MiceTransgenic OrganismsUnited StatesVirusVisual Hallucinationalpha synucleinbasecell motilitydisabilitydopaminergic neurondorsal motor nucleusfree radical oxygengain of functiongastrointestinalglucosylceramidaseinsightknock-downlocus ceruleus structureloss of functionmouse modelmutantneuropathologyolfactory bulboverexpressionpresynapticpromoterprotein aggregatepublic health relevanceresponsesmall hairpin RNAsynuclein
项目摘要
DESCRIPTION (provided by applicant): Parkinson Disease (PD) is a common, mostly sporadic neurodegenerative disease of the central and autonomic nervous systems affecting ~1 million people in the United States. Loss of substantia nigra (SN) dopaminergic neurons causes the characteristic parkinsonian movement disorder while non-motor disabilities including dementia, depression, gastrointestinal dysfunction, and autonomic instability arise from neurodegeneration in other parts of the nervous system. The cytoplasmic protein aggregates known as Lewy bodies, described nearly 100 years ago in the regions of the brain affected by PD, such as the substantia nigra, locus coeruleus, olfactory bulbs, and dorsal motor nucleus of the vagus, are considered to be the pathological hallmark of the disease. Diffuse Lewy body dementia (DLBD) accounts for ~10% of cases of all dementia, although some estimates suggest it is nearly as frequent as Alzheimer disease (AD) as a cause of dementia in the elderly. DLBD is clinically similar to AD but characteristically shows a higher frequency of psychosis with auditory and visual hallucinations and is often accompanied by the symptoms and signs of Parkinsonism. At autopsy, brains of DLBD patients have characteristic Lewy body aggregates throughout the cortex. The primary protein constituent of Lewy bodies is a-synuclein (aSyn), a 140 residue presynaptic phosphoprotein. Missense and increased copy number mutations in the gene for aSyn cause familial, autosomal dominant PD and DLBD. Since (1) aSyn-containing Lewy bodies are seen in both DLBD and PD, and (2) many of the patients in families with increased copy number mutations of aSyn have DLBD while others have PD11-15, it is very likely that the same underlying process is responsible for both conditions. The cause of most PD is unknown despite decades of research. Recently, a strong epidemological association has been found between PD/DLBD and heterozygosity for mutations in the beta-glucocerebrosidase gene (GBA), the gene in which homozygous mutations cause Gaucher disease (GD), a lysosomal storage disease. The odds ratio of finding a heterozygous GBA mutation in sporadic PD patients compared to controls ranges from the 13-28 depending on ethnic background. Thus, GBA mutations are a clear risk factor for PD but the mechanism is unknown. Being heterozygous for these mutations could increase susceptibility for PD either through haploinsufficiency of lysosomal glucocerebrosidase or through a toxic gain of function by the mutant gene product encoded by the mutant allele. The overall goal of this proposal is to develop a mouse model that uses expression of a mutant a-synuclein known to cause familial PD/DLBD to which we add either loss of function of glucocerebrosidase or expression of a mutant form of glucocerebrosidase to test for (1) changes in aSyn half-life and sensitivity to oxygen free radical challenge in cultured cells, and (2) motor and non-motor signs of PD in mice carrying pathogenic aSyn alleles and loss of function of glucocerebrosidase or expression of a mutant form of glucocerebrosidase. PUBLIC HEALTH RELEVANCE: Parkinson Disease and diffuse Lewy body dementia are common degenerative brain diseases affecting ~1 million people in the United States. The applicant wants to know if an authentic mouse model of these diseases can be created by combining genetic factors in a single mouse. These factors are the overexpression of a mutant form of 1-synuclein known to cause Parkinson disease in humans along with changes in the expression of the enzyme glucocerebrosidase, a Parkinson disease susceptibility risk factor. The ultimate goal is to create a mouse line that can be widely used to study in detail how familial Parkinson disease develops in order to identify points at which interventions could slow or stop progression of the disease.
描述(申请人提供):帕金森病(PD)是一种常见的、主要是散发性的中枢和自主神经系统退行性疾病,在美国约有100万人受到影响。黑质(SN)多巴胺能神经元的丢失会导致典型的帕金森运动障碍,而非运动障碍(包括痴呆、抑郁、胃肠功能障碍和自主神经不稳定)则是由于神经系统其他部分的神经退化引起的。近100年前,帕金森病患者脑内黑质、蓝斑、嗅球和迷走神经背侧运动核等部位的胞质蛋白聚集物被认为是帕金森病的病理特征。弥漫性路易体痴呆(DLBD)约占所有痴呆病例的10%,尽管一些估计表明,它几乎与阿尔茨海默病(AD)一样常见,也是老年人痴呆的原因之一。DLBD在临床上类似于AD,但其特点是表现出更高频率的精神病,并伴有听觉和视觉幻觉,并经常伴随帕金森氏症的症状和体征。在尸检中,DLBD患者的大脑在整个大脑皮层都有典型的路易小体聚集。路易小体的主要蛋白质成分是α-突触核蛋白(ASyn),是一种140个残基的突触前磷蛋白。ASyn基因的错义和拷贝数增加突变会导致家族性常染色体显性PD和DLBD。由于(1)在DLBD和PD中都可以看到含有a-Syn的路易体,以及(2)在aSyn拷贝数突变增加的家族中,许多患者患有DLBD,而另一些患者则患有PD11-15,因此很可能相同的潜在过程对这两种情况负责。尽管进行了数十年的研究,但大多数帕金森病的病因尚不清楚。最近,人们发现PD/DLBD与β-葡萄糖脑苷酶基因(GBA)的杂合性突变之间存在很强的流行病学联系,GBA基因的纯合突变会导致一种溶酶体储存性疾病-高谢病(GD)。散发性帕金森病患者与对照组相比,发现杂合子GBA突变的优势比从13-28不等,具体取决于种族背景。因此,GBA突变是帕金森病的一个明显的危险因素,但其机制尚不清楚。这些突变的杂合性可能会增加PD的易感性,要么是通过溶酶体葡萄糖脑苷酶单倍性不足,要么是通过突变等位基因编码的突变基因产物获得有毒功能。这项建议的总体目标是建立一种小鼠模型,利用已知导致家族性PD/DLBD的突变a-Synuclein的表达,我们在其中添加葡萄糖脑苷酶功能丧失或突变形式的葡萄糖脑苷酶的表达,以测试(1)培养细胞中aSyn半衰期的变化和对氧自由基攻击的敏感性,以及(2)携带致病aSyn等位基因的小鼠的帕金森病的运动和非运动体征,以及葡萄糖脑苷酶功能丧失或葡萄糖脑苷酶突变形式的表达。公共卫生相关性:帕金森病和弥漫性路易体痴呆是常见的退行性脑疾病,在美国约有100万人受到影响。申请者想知道是否可以通过在一只小鼠身上结合遗传因素来创建这些疾病的真实小鼠模型。这些因素是已知可导致人类帕金森病的1-突触核蛋白突变形式的过度表达,以及帕金森病易感风险因素葡萄糖脑苷酶表达的变化。最终目标是创造一种可广泛用于详细研究家族性帕金森病如何发展的小鼠系,以确定干预措施可以减缓或阻止疾病进展的点。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ROBERT L NUSSBAUM其他文献
ROBERT L NUSSBAUM的其他文献
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{{ truncateString('ROBERT L NUSSBAUM', 18)}}的其他基金
Sequencing of Newborn Blood Spot DNA to Improve and Expand Newborn Screening
新生儿血斑 DNA 测序可改善和扩大新生儿筛查
- 批准号:
8882899 - 财政年份:2013
- 资助金额:
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Sequencing of Newborn Blood Spot DNA to Improve and Expand Newborn Screening
新生儿血斑 DNA 测序可改善和扩大新生儿筛查
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Sequencing of Newborn Blood Spot DNA to Improve and Expand Newborn Screening
新生儿血斑 DNA 测序可改善和扩大新生儿筛查
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8891509 - 财政年份:2013
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$ 13万 - 项目类别:
Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
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8449266 - 财政年份:2012
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$ 13万 - 项目类别:
Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
Lowe 综合征肾病的 BAC 转基因和敲除小鼠模型相结合
- 批准号:
8236723 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
Lowe 综合征肾病的 BAC 转基因和敲除小鼠模型相结合
- 批准号:
8627601 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
GI Endotoxin as an Environmental Trigger in an alpha-Synuclein Transgenic Mouse
胃肠道内毒素作为 α-突触核蛋白转基因小鼠的环境触发因素
- 批准号:
8305727 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
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- 批准号:
8081385 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
GI Endotoxin as an Environmental Trigger in an alpha-Synuclein Transgenic Mouse
胃肠道内毒素作为 α-突触核蛋白转基因小鼠的环境触发因素
- 批准号:
8107578 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
GI Endotoxin as an Environmental Trigger in an alpha-Synuclein Transgenic Mouse
胃肠道内毒素作为 α-突触核蛋白转基因小鼠的环境触发因素
- 批准号:
7985599 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
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