Structural studies of Bacillus thuringiensis Cry Toxins
苏云金芽孢杆菌 Cry 毒素的结构研究
基本信息
- 批准号:7751274
- 负责人:
- 金额:$ 14.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-12-19 至 2011-11-30
- 项目状态:已结题
- 来源:
- 关键词:Adrenergic ReceptorAlkanesulfonatesAmyloidBacillus thuringiensisBacterial ToxinsBacteriorhodopsinsBiochemicalBiologicalBiological ModelsCationsCellsCessation of lifeCryingCrystallizationCulicidaeDehydrationDetergentsDiseaseDisease VectorsEngineeringEquilibriumGrantHealthHumanHydroxyl RadicalIncidenceIngestionInsectaInsecticidesIon ChannelKnowledgeLaboratoriesLipidsMalariaMalnutritionMembraneMembrane LipidsMembrane ProteinsMethodsMidgutModelingMolecular ConformationNematodaOrder ColeopteraPhosphorylcholinePropanePropertyProteinsProteolytic ProcessingReproduction sporesResolutionRiskRoleScreening procedureStructureSystemTestingThermodynamicsToxinVariantchapsodisease transmissionimprovedinsightinterestkillingsmosquitocidalmutantnoveloverexpressionpractical applicationprotein protein interactionprotein structurepublic health relevancereceptorvector mosquito
项目摘要
DESCRIPTION (provided by applicant): The Bacillus thuringiensis Cry proteins are novel bacterial toxins with practical application as biological insecticides for the targeting of specific crop pests (caterpillars, beetles, nematodes) and disease vectors (mosquitoes). Insect ingestion of B. thuringiensis crystals [or spores and crystals] leads to solublization and proteolytic processing of Cry proteins that are thought to oligomerize and then insert into the midgut cells of insect. In the leading model for mechanism of action, this insertion results in the formation of cation-specific ion channels that collapse the electrochemical gradient of the midgut cells and ultimately leads to insect death. One important step towards understanding how Cry proteins insert into membranes would be the availability of Cry protein structures in both their soluble and membrane-inserted forms. Crystallographic efforts have provided structures of several Cry protein variants in their soluble forms, but as yet no structure of a Cry protein in its membrane inserted-form has been determined. This R21 grant will explore strategies towards the structural determination of such a membrane-inserted Cry toxin. PUBLIC HEALTH RELEVANCE: The Bacillus thuringiensis Cry proteins are novel bacterial toxins with practical application as biological insecticides for the specific targeting of insect crop pests and disease vectors. This proposal seeks to determine the structure of Cry toxins in the form that kills insects, knowledge that could be used to generate improved toxins. Such super Cry toxins could be used to improve human health by increasing crop yields, thereby helping to reduce human malnutrition, and by killing mosquitoes that can transmit diseases, resulting in lower incidence of sickness.
描述(由申请人提供):苏云金芽孢杆菌Cry蛋白是新型细菌毒素,其实际应用为靶向特定作物害虫(毛虫、甲虫、线虫)和疾病媒介(蚊子)的生物杀虫剂。昆虫吞食B。苏云金芽孢杆菌晶体[或孢子和晶体]导致Cry蛋白的溶解和蛋白水解加工,Cry蛋白被认为寡聚化,然后插入昆虫的中肠细胞。在作用机制的主要模型中,这种插入导致形成阳离子特异性离子通道,这些离子通道破坏中肠细胞的电化学梯度,最终导致昆虫死亡。理解Cry蛋白如何插入膜的一个重要步骤是Cry蛋白结构以其可溶性和膜插入形式的可用性。晶体学研究已经提供了几种可溶形式的Cry蛋白变体的结构,但尚未确定其膜插入形式的Cry蛋白的结构。这项R21赠款将探索这种膜插入Cry毒素的结构确定策略。公共卫生相关性:苏云金芽孢杆菌(Bacillus thuringiensis)Cry蛋白是一种新型的细菌毒素,具有作为生物杀虫剂的实际应用,用于特异性靶向昆虫作物害虫和疾病媒介。该提案旨在确定杀死昆虫的Cry毒素的结构,这些知识可用于产生改进的毒素。这种超级Cry毒素可以通过增加作物产量来改善人类健康,从而帮助减少人类营养不良,并通过杀死可以传播疾病的蚊子来降低疾病发生率。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael K Chan其他文献
Cry3AaSpyCatcher fusion crystals produced in bacteria as scaffolds for multienzyme co-immobilization
细菌中产生的 Cry3AaSpyCatcher 融合晶体作为多酶共固定的支架
- DOI:
10.1021/acs.bioconjchem.2c00003 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
Qian Sun;Bradley S Heater;Tin Lok Li;Weijian Ye;Zhihong Guo;Michael K Chan - 通讯作者:
Michael K Chan
Michael K Chan的其他文献
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{{ truncateString('Michael K Chan', 18)}}的其他基金
OSU Crystallography Facility: Acquisition of Crystallization Robot
OSU 晶体学设施:购置结晶机器人
- 批准号:
7794649 - 财政年份:2010
- 资助金额:
$ 14.85万 - 项目类别:
STRUCTURAL BIOLOGY OF SMALL MOLECULE SENSORS, CHANNELS AND ACTIVATORS
小分子传感器、通道和激活剂的结构生物学
- 批准号:
8170080 - 财政年份:2010
- 资助金额:
$ 14.85万 - 项目类别:
STRUCTURAL BIOLOGY OF SMALL MOLECULE SENSORS, CHANNELS AND ACTIVATORS
小分子传感器、通道和激活剂的结构生物学
- 批准号:
7954407 - 财政年份:2009
- 资助金额:
$ 14.85万 - 项目类别:
L-pyrrolysine Chemical and Structural Biology
L-吡咯赖氨酸化学和结构生物学
- 批准号:
7923567 - 财政年份:2009
- 资助金额:
$ 14.85万 - 项目类别:
STRUCTURAL BIOLOGY OF SMALL MOLECULE SENSORS, CHANNELS AND ACTIVATORS
小分子传感器、通道和激活剂的结构生物学
- 批准号:
7722098 - 财政年份:2008
- 资助金额:
$ 14.85万 - 项目类别:
Structural studies of Bacillus thuringiensis Cry Toxins
苏云金芽孢杆菌 Cry 毒素的结构研究
- 批准号:
7573883 - 财政年份:2008
- 资助金额:
$ 14.85万 - 项目类别: