SAXS STUDIES OF METALLOPROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 (1)MtmB-MtmC复合体-我们能够收集关于MtmB复合体的数据，该复合体由六个MtmB亚单位(6 X 60 KDa)与6个MtmC单位(29 KDa)结合组成。他们能够为该建筑群获得合理的数据。我们希望通过收集MtmB六聚体的数据来完成这项研究，以便我们能够更好地识别结构差异。我们有MtmB六聚体和MtmC的晶体结构，并试图将数据与MS研究相关联，这些研究也在进行中。另一个值得注意的特征是，MtmB含有第22个遗传编码的氨基酸吡咯赖氨酸，用于激活底物向MtmC的转移。(2)嗜热嗜热杆菌羧基肽酶-我们在JBC上有一篇关于该蛋白质晶体结构的修订论文。作为修订的一部分，他们要求我们验证其在溶液中的二聚性。对相关体系的凝胶过滤得到反常结果为83 kDa，而单体为59 kDa，二聚体为118 kDa。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (1) MtmB-MtmC complex - We were able to collect data on the MtmB complex comprised of a hexamer of MtmB subunits (6 x 60 kDa) bound to six MtmC units (29 kDa). They were able to get reasonable data for the complex. We would like to complete this study by collecting data on the MtmB hexamer alone so that we can better identify the structural differences. We have crystal structures of the MtmB hexamer and MtmC and are trying to correlate the data with MS studies also ongoing. Another notable feature is that MtmB contains the 22nd genetically encoded amino acid pyrrolysine which is used to activate substrate transfer to MtmC. (2) Thermus thermophilus carboxypeptidase -We have a paper in revision at JBC on the crystal structure of this protein. As part of the revision, they have asked us to verify its dimerization in solution. Gel filtration on related systems give an anomalous result of 83 kDa compared to the monomer 59 kDa and dimer 118 kDa.