UNDERSTANDING MECHANISMS OF hESC SELF-RENEWAL AND CELL FATE COMMITMENT

该计划的主要主题将是确定 人类胚胎干细胞的基本自我更新机制以及它们如何分化为不同的谱系。每个人 的研究项目是互补的，但具有高度的协同性，并建立在共同的生物学基础上 站台。该计划非常重视使用最新的hESC培养技术，并将 与东南科学界高度互动。这3个项目将：-解决分子 以及早期细胞命运承诺的细胞方面；-决定基因组在 -并应用最先进的技术来表征hESC和hESC的糖基化模式 分化的细胞。三个核心将：-作为计划的资源，东南By 维护和分发hesc，提供hesc为期两周的强化培训计划 技术并作为一般技术资源发挥作用；-建立旨在 解决制约hesc领域进展的主要问题；--提供行政、后勤支持 作为与东南部互动的渠道。该计划将显著提高 加快佐治亚大学和合作伙伴在hESC领域取得的科学进展 东南部的机构。总体而言，该团队的发现将对我们的 了解人类胚胎干细胞生物学和人类发展的基础知识。所有这些发现都将是至关重要的 用于制定将人类胚胎干细胞用于细胞治疗的策略 退行性疾病和慢性损伤的修复。

The overarching theme of this Program will be to define basic self-renewal mechanisms of hESCs and how they become specified into different lineages. Each of the research projects is complementary but highly synergistic and built on a common biological platform. The Program places a strong emphasis on using the latest hESC culture technology and will be highly interactive with the Southeast scientific community. The 3 Projects will: - address molecular and cellular aspects of early cell fate commitment; - determine how the genome is remodeled during differentiation;- and apply state of the art technology to characterize glycosylation patterns in hESC and differentiated cells. The 3 Cores will: - function as a resource for the Program and the Southeast by maintaining and distributing hESCs, providing an intensive 2-week Training Program in hESC technology and functioning as a general technical resource; -establish new technologies designed to solve major problems that restrict progress in the hESC area; - provide administrative, logistical support for the Program and act as a conduit for interactions with the Southeast. The Program will significantly accelerate scientific advances made in the hESC area at the University of Georgia and at partnering Institutions in the Southeast. Overall, discoveries made by this team will have a major impact on our understanding of basic hESC biology and human development. All of these discoveries will be critical for the development of strategies where hESCs are to be used in cell therapies for treating degenerative disease and repair of chronic injury.