UNDERSTANDING MECHANISMS OF hESC SELF-RENEWAL AND CELL FATE COMMITMENT

产品说明：（申请人提供）：该计划的首要主题将是定义hESC的基本自我更新机制以及它们如何被指定为不同的谱系。每个研究项目都是互补的，但高度协同，并建立在一个共同的生物平台上。该计划非常重视使用最新的hESC培养技术，并将与东南科学界高度互动。 这三个项目将： - 解决早期细胞命运定型的分子和细胞方面; - 确定基因组在分化过程中如何重塑;以及 - 应用最先进的技术来表征hESC中的糖基化模式， 分化细胞 三个核心将： - 通过维护和分配hESC，提供为期2周的hESC技术强化培训计划，并作为一般技术资源，为该计划和东南部提供资源; - 开发新技术，以解决限制人胚胎干细胞领域进展的主要问题; - 为该计划提供行政和后勤支持，并充当与东南部互动的渠道。 该计划将大大加快在hESC领域取得的科学进步在格鲁吉亚大学和在东南部的合作机构。总的来说，该团队的发现将对我们对基本hESC生物学和人类发育的理解产生重大影响。所有这些发现对于开发hESC用于治疗退行性疾病和修复慢性损伤的细胞疗法的策略至关重要。

DESCRIPTION: (provided by applicant): The overarching theme of this Program will be to define basic self-renewal mechanisms of hESCs and how they become specified into different lineages. Each of the research projects is complementary but highly synergistic and built on a common biological platform. The Program places a strong emphasis on using the latest hESC culture technology and will be highly interactive with the Southeast scientific community. The 3 Projects will: - address molecular and cellular aspects of early cell fate commitment; - determine how the genome is remodeled during differentiation;- and - apply state of the art technology to characterize glycosylation patterns in hESC and differentiated cells. The 3 Cores will: - function as a resource for the Program and the Southeast by maintaining and distributing hESCs, providing an intensive 2-week Training Program in hESC technology and functioning as a general technical resource; -establish new technologies designed to solve major problems that restrict progress in the hESC area; - provide administrative, logistical support for the Program and act as a conduit for interactions with the Southeast. The Program will significantly accelerate scientific advances made in the hESC area at the University of Georgia and at partnering Institutions in the Southeast. Overall, discoveries made by this team will have a major impact on our understanding of basic hESC biology and human development. All of these discoveries will be critical for the development of strategies where hESCs are to be used in cell therapies for treating degenerative disease and repair of chronic injury.