CLINICAL TRIAL: INVASIVE ASPERGILLOSIS DIAGNOSIS AND PATHOGENESIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 侵袭性曲霉病是一种真菌感染，主要影响免疫功能低下的患者。 大多数侵袭性曲霉病是由烟曲霉引起的。 目前迫切需要确定新的抗真菌治疗靶点，以防止这种微生物感染人类。 本研究的目的是建立临床相关的实验模型，以研究A。烟曲霉引起感染。 作为这些研究的一部分，我们计划研究A.具有内皮细胞的烟曲霉。 这些细胞形成血管的内层。 本研究的长期目标是了解A。烟曲霉侵入、破坏并激活这些细胞，因此可以开发阻断该过程的方法。 这项工作是重要的，因为侵袭性曲霉病患者的死亡率约为50%，即使目前可用的治疗。 对于拟定的研究，将从人脐带内的静脉中收获内皮细胞，并在组织培养物中生长。 脐带将在正常阴道分娩后从未感染的供体中获得。 不会收集关于母亲或婴儿的任何信息。 脐带血是由脐带血中的一种成分所产生的，是由脐带血中的一种成分所产生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Invasive aspergillosis is a fungal infection that primarily affects immunocompromised patients. Most cases of invasive aspergillosis are caused by Aspergillus fumigatus. There is a critical need for the identification of novel targets for antifungal therapies that can prevent this organism from infecting people. The purpose of this research project is to develop clinically relevant experimental models to study the mechanisms by which A. fumigatus causes infection. As part of these investigations, we plan to study the interactions of A. fumigatus with endothelial cells. These cells form the inner lining of the blood vessels. The long-range goal of this research is to understand how A. fumigatus invades, damages, and activates these cells so that methods to block this process can be developed. This work is important because the mortality of patients with invasive aspergillosis is approximately 50%, even with currently available therapy. For the proposed studies, the endothelial cells will be harvested from the veins within human umbilical cords and grown in tissue culture. The umbilical cords will be obtained after normal vaginal births from uninfected donors. No information about the mother or baby will be collected. The donors of the cords will not be identified and the umbilical cord blood will not be collected.