TRANSCRIPTIONAL REGULATION OF A FUMIGATUS VIRULENCE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究的目的是研究真菌烟曲霉与内皮细胞的相互作用。这些细胞形成血管的内层。这项研究的长期目标是了解烟曲霉菌是如何入侵、刺激和破坏这些细胞的，以便开发出阻止这一过程的方法。这项工作很重要，因为即使在目前可用的治疗方法下，播散性念珠菌感染患者的死亡率也约为50%。在拟议的研究中，内皮细胞将从人脐带内的静脉中采集，并在组织培养中生长。脐带将在未感染的捐赠者正常阴道分娩后获得。不会收集有关母亲或婴儿的信息。脐带捐赠者的身份将不会被识别，脐带血也不会被收集。脐带将在港湾-加州大学洛杉矶分校医疗中心和锡达斯-西奈医疗中心收集。本研究还将研究烟曲霉菌与A549 2型肺泡细胞株的相互作用。该细胞系最初是1972年从一名肺癌患者身上分离出来的。它将从美国典型培养物收藏中获得。没有任何信息将这名患者的身份与细胞系联系起来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is study the interactions of the fungus, Aspergillus fumigatus, with endothelial cells. These cells form the inner lining of the blood vessels. The long-range goal of this research is to understand how A. fumigatus invades, stimulates, and damages these cells so that methods to block this processes can be developed. This work is important because the mortality of patients with a disseminated candidal infection is approximately 50%, even with currently available therapy. For the proposed studies, the endothelial cells will be harvested from the veins within human umbilical cords and grown in tissue culture. The umbilical cords will be obtained after normal vaginal births from uninfected donors. No information about the mother or baby will be collected. The donors of the cords will not be identified and the umbilical cord blood will not be collected. The umbilical cords will collected at both Harbor-UCLA Medical Center and at Cedars-Sinai Medical Center. This study will also study the interactions of A. fumigatus with the A549 type 2 pneumocyte cell line. This cell line was originally isolated from a human with lung cancer in 1972. It will be obtained from the American Type Culture Collection. There is no information linking the identity of the this patient with the cell line.