TRANSCRIPTIONAL REGULATION OF A FUMIGATUS VIRULENCE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是研究真菌烟曲霉与内皮细胞的相互作用。 这些细胞形成血管的内层。 本研究的长期目标是了解A。烟曲霉侵入、刺激和损害这些细胞，因此可以开发阻断该过程的方法。 这项工作很重要，因为即使采用目前可用的治疗方法，播散性念珠菌感染患者的死亡率也约为50%。 对于拟定的研究，将从人脐带内的静脉中收获内皮细胞，并在组织培养物中生长。 脐带将在正常阴道分娩后从未感染的供体中获得。 不会收集关于母亲或婴儿的任何信息。 脐带血是由脐带血中的一种成分所产生的，是由脐带血中的一种成分所产生。 脐带将在Harbor-UCLA医疗中心和Cedars-Sinai医疗中心收集。 本研究还将研究A.烟曲霉与A549 2型肺细胞系。 该细胞系最初于1972年从患有肺癌的人中分离出来。 将从美国典型培养物保藏中心获得。 没有将该患者的身份与细胞系联系起来的信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this research project is study the interactions of the fungus, Aspergillus fumigatus, with endothelial cells. These cells form the inner lining of the blood vessels. The long-range goal of this research is to understand how A. fumigatus invades, stimulates, and damages these cells so that methods to block this processes can be developed. This work is important because the mortality of patients with a disseminated candidal infection is approximately 50%, even with currently available therapy. For the proposed studies, the endothelial cells will be harvested from the veins within human umbilical cords and grown in tissue culture. The umbilical cords will be obtained after normal vaginal births from uninfected donors. No information about the mother or baby will be collected. The donors of the cords will not be identified and the umbilical cord blood will not be collected. The umbilical cords will collected at both Harbor-UCLA Medical Center and at Cedars-Sinai Medical Center. This study will also study the interactions of A. fumigatus with the A549 type 2 pneumocyte cell line. This cell line was originally isolated from a human with lung cancer in 1972. It will be obtained from the American Type Culture Collection. There is no information linking the identity of the this patient with the cell line.