GEN-RED

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这是合作的R01四年竞争延续提案的第二次修订，该提案创建了一个基于存储库的复发、早发性严重抑郁障碍病例的大型样本，并使用位置克隆来识别我们基因组扫描中显著关联的区域中的抑郁症易感基因。完成的四年项目收集了680个家庭，其中包括927对受影响的兄弟姐妹对和MDD-RE诊断模型以及其他受影响的亲属。用于细胞培养的盲法临床数据和血液样本已保存在NIMH储存库中，并正在公布。连锁精细定位在染色体15q上发现了全基因组范围的显著连锁；在10 cM基因组扫描中，在#40；6p-q、8p、17p和#41；三个区域观察到了暗示的性别特异连锁，染色体17p上的结果接近全基因组意义。六个合作网站现在提议：在第一至第三年收集1,350个符合相同标准的欧洲血统MDD-RE先证者，包括有受影响的兄弟姐妹的证据，以创建总共2,000欧元MDD-RE病例的资料库样本，以及来自可获得的父母、未受影响的兄弟姐妹和男性-男性ASP的细胞系/DMA。启动基于资料库的符合相同临床标准的非裔美国人MDD-RE先证者的集合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a second revision of a collaborative R01 four-year competing continuation proposal to create a large repository-based sample of cases with recurrent, early-onset major depressive disorder (MDD-RE), and to use positional cloning to identify depression susceptibility genes in regions of significant linkage in our genome scan. The completed four-year project collected 680 families containing 927 affected sibling pairs (ASPs) (MDD-RE diagnostic model) and additional affected relatives (GenRED I). Blinded clinical data and blood specimens for cell culture were deposited in the NIMH repository and are being made public. Linkage fine-mapping has demonstrated genome-wide significant linkage on chromosome 15q; in the 10 cM genome scan, suggestive sex-specific linkage was observed in three regions (6p-q, 8p, 17p), with the result on chromosome 17p approaching genome-wide significance. Six collaborating sites now propose to: (1) Collect (during Years 1-3) an additional 1,350 European-ancestry (EUR) MDD-RE probands (GenRED II) meeting identical criteria (including evidence of having an affected sibling) to create a total repository sample of 2,000 EUR MDD-RE cases, plus cell lines/DMA from available parents, unaffected sibs and male-male ASPs. (2) Initiate a repository-based collection of African-American (AA) MDD-RE probands meeting the same clinical criteria.