GEN BD

基因BD

• 批准号: 7607818
• 负责人: WILLIAM Bradford LAWSON
• 金额: $ 15.46万
• 依托单位: HOWARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607818
• 关键词: 10p; 13q; 16p; 17q; 21q; 22q; African American; Area; Bioinformatics; Bipolar Disorder; Cell Line; Chromosomes; Clinical Data; Computer Retrieval of Information on Scientific Projects Database; Contracts; DNA; Data Linkages; Disease; Family; Funding; Genes; Genetic; Genomics; Genotype; Goals; Grant; Individual; Inherited; Institution; Interview; Laboratories; Linkage Disequilibrium; Maps; National Institute of Mental Health; Parents; Polymorphic Microsatellite Marker; Publishing; Research; Research Personnel; Resources; SNP genotyping; Sampling; Site; Source; Standards of Weights and Measures; Stratification; Techniques; United States National Institutes of Health; Universities; base; case control; design; follow-up; genetic pedigree; genetic resource; genome wide association study; proband

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Since 1988 the NIMH Genetics Initiative has supported a national resource for the study of bipolar disorder (BP). By 1997 153 multiplex families were assessed, providing cell lines, DNA, and anonymized clinical data. This is now a publicly available resource and analytic results have been published. A second effort commenced in 1998 to ascertain 500 new BP sib pairs; this goal has been exceeded with 523 additional BPI sib pairs ascertained, interviewed, and a DNA sample collected. A genome wide scan has been completed at the Center for Inherited Disease Research (CIDR) on 237 sib pair families; the remaining 309 families will be genotyped by CIDR during 2003. This resource, the largest of its kind, has revealed evidence for area of linkage on chromosomes 6q and 17q. It has also provided confirmation of a locus on chromosome 22q and support for areas on 1p, 10p, 16p, 13q, and 21q. Accumulating linkage data has implicated other chormosomal regions. We propose an extension of the national genetic resource to include a sample of 5000 unrelated BP probands and 2000 parents for case-control and family-based association resource. Probands and parents will be ascertained and assessed at eleven sites (the ten sites previously participating plus Howard University, which will provide additional African-American probands). This sample will be a national resource for fine scale linkage disequilibrium mappping within regions of linkage, as well as condidate gene association studies. Parental DNAs in a subsample will allow additional control for ethnic stratification. Bioinformatics techniques will be developed and supported for genomic analysis of condidate regions, to assist selection of SNPs and other polymorphic markers and primer design. The genotyping will be coordinated across 8 labs with an informed step-wise approach, beginning with standard micro-satellite mapping of the current set of 699 pedigrees, followed by contract genotyping of SNPs in an industrial laboratory, and continuing with follow-up genotyping of regions and sequencing of associated genes in laboratories at the individual sites. SNP typing of the larger case-control sample will occur primarily in the final year of the collaborative study. Analysis of the existing sib pair families plus this large set of cases and controls should permit the confirmation of several vulnerability genes during this grant period.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 自1988年以来，NIMH遗传学倡议支持了躁郁症研究的国家资源（BP）。 到1997年，评估了153个多重家族，提供细胞系，DNA和匿名临床数据。 现在，这是一种公开可用的资源，并且已经发布了分析结果。 1998年开始了第二次努力，以确定500个新的BP SIB对；该目标已超过523个BPI SIB对确定，采访和收集的DNA样品。 基因组广泛的扫描已在遗传性疾病研究中心（CIDR）对237个SIB对家族进行；剩下的309个家庭将由CIDR在2003年期间进行基因分型。此类资源是同类的最大资源，它揭示了染色体6Q和17q上连锁区域的证据。 它还提供了对22q染色体染色体上的基因座的确认，并为1p，10p，16p，13q和21q的面积提供了支持。 累积的链接数据牵涉到其他cal体形区域。 我们建议对国家遗传资源的扩展，包括5000个无关的BP Probands和2000年父母的样本，以进行病例对照和基于家庭的关联资源。 将在11个地点（以前参与的十个站点加霍华德大学，将提供额外的非裔美国人证据），确定和评估父母。 该样本将是在连锁区域内进行精细链接不平衡映射的国家资源，以及凝固基因关联研究。 子样本中的父母DNA将允许种族分层的额外控制。 将开发和支持生物信息学技术，用于凝固区域的基因组分析，以帮助选择SNP和其他多态标记和引物设计。 该基因分型将在8个实验室中以知情的阶梯方法进行协调，从标准的微型卫星图映射开始，对当前的699个血统组的标准微观映射开始，然后是工业实验室中SNP的合同基因分型，然后继续以各个位置的实验室中的区域和测序进行后续基因分型。 较大病例对照样本的SNP键入将主要发生在协作研究的最后一年。 对现有的SIB对家族的分析以及此大量病例和控制措施应允许在此赠款期间确认几个漏洞基因。