CLINICAL TRIAL: DIET, EXERCISE, AND METABOLISM IN OLDER WOMEN (DEMO)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 有氧运动（AEX）强度可能是一个重要的因素，影响选择性损失腹部脂肪时，结合低热量饮食（DIET）。 本研究的目的是确定细胞机制，AEX强度影响腹部脂肪组织损失的条件下，同样的能量不足的绝经后妇女腹部肥胖。 假设是，与单独的饮食或低强度AEX饮食相比，高强度AEX饮食将增加腹部脂肪的损失和代谢性心血管疾病（CVD）风险因素的改善，这是由于脂蛋白脂肪酶（LPL）活性的更大降低，以及腹部脂肪组织相对于臀部脂肪组织的脂解作用的更大增加。 我们还将测量可能参与区域脂肪储存调节的基因的信使核糖核酸（mRNA）水平，以开始确定不同强度的AEX饮食如何影响基因表达以改变体脂分布。通过不同强度的AEX和不AEX的饮食影响甘油三酯的区域摄取和动员以改变体脂分布的机制的鉴定，对于开发最有效的治疗方法以促进腹部脂肪的优先损失和改善腹部肥胖女性的CVD代谢危险因素具有重要的临床意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aerobic exercise (AEX) intensity may be an important factor affecting the selective loss of abdominal fat when combined with a hypocaloric diet (DIET). The goal of this study is to determine the cellular mechanisms by which AEX intensity affects the loss of abdominal adipose tissue under conditions of equal energy deficit in postmenopausal women with abdominal obesity. The hypothesis is that, compared to DIET alone or DIET with low-intensity AEX, DIET with high-intensity AEX will augment the loss of abdominal fat and improvements in metabolic cardiovascular disease (CVD) risk factors due to greater reductions in lipoprotein lipase (LPL) activity, and to greater increases in lipolysis, in abdominal, relative to gluteal, adipose tissue. We also will measure messenger ribonucleic acid (mRNA) levels of genes that may be involved in the regulation of regional fat storage to begin to determine how DIET with and without AEX of different intensities affects gene expression to alter body fat distribution. Identification of the mechanisms by which DIET with and without AEX of different intensities affects regional uptake and mobilization of triglyceride to alter body fat distribution will have important clinical implications for the development of the most effective treatment to promote the preferential loss of abdominal fat and improve CVD metabolic risk factors in women with abdominal obesity.