CLINICAL TRIAL: DIET, EXERCISE, AND METABOLISM IN OLDER WOMEN (DEMO)

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 有氧运动(AEX)强度可能是一个重要因素，当与低热量饮食(饮食)相结合时，会影响腹部脂肪的选择性损失。本研究的目的是在绝经后腹型肥胖妇女等能量缺乏的条件下，确定AEX强度影响腹部脂肪组织丢失的细胞机制。假设是，与单独饮食或低强度AEX饮食相比，高强度AEX饮食将增加腹部脂肪的损失，并改善代谢性心血管疾病(CVD)风险因素，这是由于脂蛋白脂酶(LPL)活性的更大降低，以及腹部相对于臀部、脂肪组织的脂肪分解的更大增加。我们还将测量可能参与区域脂肪储存调控的基因的信使核糖核酸(MRNA)水平，以开始确定添加和不添加不同强度的AEX的饮食如何影响基因表达，从而改变体内脂肪分布。明确不同强度的AEX饮食对局部摄取和动员甘油三酯以改变体内脂肪分布的影响机制，对于开发最有效的治疗腹型肥胖女性腹部脂肪优先损失和改善心血管代谢危险因素的方法具有重要的临床意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aerobic exercise (AEX) intensity may be an important factor affecting the selective loss of abdominal fat when combined with a hypocaloric diet (DIET). The goal of this study is to determine the cellular mechanisms by which AEX intensity affects the loss of abdominal adipose tissue under conditions of equal energy deficit in postmenopausal women with abdominal obesity. The hypothesis is that, compared to DIET alone or DIET with low-intensity AEX, DIET with high-intensity AEX will augment the loss of abdominal fat and improvements in metabolic cardiovascular disease (CVD) risk factors due to greater reductions in lipoprotein lipase (LPL) activity, and to greater increases in lipolysis, in abdominal, relative to gluteal, adipose tissue. We also will measure messenger ribonucleic acid (mRNA) levels of genes that may be involved in the regulation of regional fat storage to begin to determine how DIET with and without AEX of different intensities affects gene expression to alter body fat distribution. Identification of the mechanisms by which DIET with and without AEX of different intensities affects regional uptake and mobilization of triglyceride to alter body fat distribution will have important clinical implications for the development of the most effective treatment to promote the preferential loss of abdominal fat and improve CVD metabolic risk factors in women with abdominal obesity.