ANTIOXIDANT STATUS, DIET, AND EARLY PREGNANCY

抗氧化状态、饮食和怀孕早期

• 批准号: 7951339
• 负责人: Terryl J. Hartman
• 金额: $ 0.83万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951339
• 关键词: 8-hydroxy-2' -deoxyguanosine; Antioxidants; Ascorbic Acid; Biochemical Reaction; Biological Assay; Biological Markers; Carotenoids; Clinical Research; Cohort Studies; Computer Retrieval of Information on Scientific Projects Database; Conceptions; Contraceptive methods; Diet; Diet Records; Dietary Practices; Embryo; Epidemiologic Studies; Failure; Funding; Goals; Grant; Human; Human Chorionic Gonadotropin; Institution; Isoprostanes; Maternal Exposure; Measures; Menstrual cycle; Oocytes; Outcome; Ovulation; Oxidative Stress; Planned Pregnancy; Pre-Eclampsia; Pregnancy; Pregnancy loss; Reactive Oxygen Species; Reproduction; Research; Research Personnel; Resources; Serum; Source; Spontaneous abortion; Time; United States National Institutes of Health; Woman; egg; men; nutrition; preterm premature rupture of membranes; prospective; reproductive; sperm function; urinary

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human reproduction is inefficient with less than one-third of fertilized human eggs likely to survive to produce a term delivery. The great majority of these failures occur in the early weeks after ovulation. The causes of these reproductive failures are substantially unknown, but evidence suggests that they may be a consequence of maternal exposures, including pre-conception nutrition, that may result in insults to the oocyte and periconceptual embryo. The goal of this prospective epidemiologic study is to evaluate the hypothesis that dietary patterns associated with increased oxidative stress result in delayed time-to-pregnancy or early loss. Reactive oxygen species (ROS) have been associated with spontaneous abortion, preeclampsia, and premature preterm rupture of the membranes in women and defective sperm function in men. ROS are formed continuously as a consequence of both biochemical reactions and external factors. We propose to conduct a cohort study of 100 healthy women planning pregnancy. The outcomes to be evaluated are time-to-pregnancy, defined as the number of menstrual cycles from the cessation of contraception to a clinically recognized pregnancy, and early, unrecognized pregnancy loss. Unrecognized pregnancy and subsequent early loss will be determined by measuring urinary human chorionic gonadotropin (hCG). Diet will be assessed prior to conception using diet records and serum assays for the antioxidant vitamins C and E, and the carotenoids. Two urinary biomarkers of oxidative stress, 8-hydroxy-2'-deoxyguanosine and F2¿-isoprostanes will be measured.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 人类繁殖效率低下，只有不到三分之一的受精卵能够存活并足月分娩。 大多数失败发生在排卵后的最初几周。 这些生殖失败的原因基本上未知，但有证据表明，它们可能是母体暴露的结果，包括受孕前的营养，这可能会导致卵母细胞和围孕期胚胎受到损害。 这项前瞻性流行病学研究的目的是评估以下假设：与氧化应激增加相关的饮食模式会导致怀孕时间延迟或早期流产。 活性氧 (ROS) 与女性自然流产、先兆子痫、胎膜早破和男性精子功能缺陷有关。 ROS 由于生化反应和外部因素而不断形成。 我们建议对 100 名计划怀孕的健康女性进行队列研究。 要评估的结果是怀孕时间，定义为从停止避孕到临床认可的妊娠的月经周期数，以及早期、未被识别的妊娠丢失。 未识别的妊娠和随后的早期流产将通过测量尿人绒毛膜促性腺激素 (hCG) 来确定。 在受孕前，将使用饮食记录和抗氧化剂维生素 C 和 E 以及类胡萝卜素的血清检测来评估饮食。 将测量氧化应激的两种尿液生物标志物：8-羟基-2'-脱氧鸟苷和F2-异前列腺素。