CHARACTERIZATION OF PROLIFERATING COMPARTMENT IN B-CELL CLL PATIENTS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 我们建议： 1. 继续确定来自骨髓和血液的未治疗患者的B-CLL细胞的动力学特征，以鉴定B-CLL中的增殖细胞，并将这些数据与B-CLL细胞的特定特征、临床病程和各种可用的预后标志物相关联。 在某些情况下，将重新研究先前分析白血病细胞动力学的受试者，以确定出生率和死亡率是否发生变化。 2. 确定健康老年受试者正常血液B细胞亚群的动力学特征。 这样做的原因是为了确定与50岁或以上的衰老相关的任何环境因素。 3. 将B-CLL细胞的动力学特征与在一些正常衰老个体和未受影响的家庭成员（B-CLL患者的遗传信息亲属）中可检测到的B细胞克隆扩增的动力学特征进行比较。 我们正在寻找18岁或以上的遗传相似的成年人，以确定与CLL发展相关的因素以及与衰老相关的环境因素。 4. 分析增殖的B-CLL细胞和来自老年个体和未受影响的家庭成员（其是B-CLL患者的遗传信息亲属）的克隆扩增的正常B细胞，以确定是否存在不同于静息B-CLL池的细胞遗传学异常。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to: 1. Continue to determine the kinetic profiles of B-CLL cells of untreated patients, from the bone marrow and the blood, to identify the proliferating cells in B-CLL and to correlate these data with specific features of B-CLL cells, clinical course, and the various available prognostic markers. In some instances, subjects previously analyzed for leukemic cell kinetics will be re-studied to determine if birth and death rates have changed. 2. Determine the kinetic profiles of normal blood B cell subsets from healthy aging subjects. The reason for this is to determine any environmental factors that are associated with aging, 50 years or older. 3. Compare the kinetic profiles of B-CLL cells with those of B cell clonal amplifications detectable in some normal aging individuals and unaffected family members who are genetically informative relatives of B-CLL patients. We are looking for genetically similar adults 18 years or older to determine factors that are associated with the development of CLL as well as environmental factors that are associated with aging. 4. Analyze the proliferating B-CLL cells, and the clonally expanded normal B cells from elderly individuals and unaffected family members who are genetically informative relatives of B-CLL patients, for the presence of cytogenetic abnormalities that differ from those of the resting B-CLL pool.