CHARACTERIZATION OF PROLIFERATING COMPARTMENT IN B-CELL CLL PATIENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议： 1.继续测定未治疗患者骨髓和血液中B-CLL细胞的动力学特征，以确定B-CLL中的增殖细胞，并将这些数据与B-CLL细胞的特殊特征、临床病程和各种可用的预后标记物相关联。在某些情况下，以前对白血病细胞动力学进行分析的受试者将被重新研究，以确定出生和死亡率是否发生了变化。 2.测定健康老年人外周血中正常B细胞亚群的动态变化。这样做的原因是为了确定与衰老有关的任何环境因素，50岁或以上。 3.将B-CLL细胞的动力学特征与在一些正常老年人和B-CLL患者的遗传信息相关的未受影响的家庭成员中可检测到的B细胞克隆扩增的动力学特征进行比较。我们正在寻找基因相似的18岁或以上的成年人，以确定与CLL的发展相关的因素，以及与衰老相关的环境因素。 4.分析正在增殖的B-CLL细胞，以及来自老年人和未受影响的家庭成员的克隆性扩增的正常B细胞，他们是B-CLL患者的遗传信息丰富的亲属，以确定是否存在与静止的B-CLL池不同的细胞遗传学异常。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to: 1. Continue to determine the kinetic profiles of B-CLL cells of untreated patients, from the bone marrow and the blood, to identify the proliferating cells in B-CLL and to correlate these data with specific features of B-CLL cells, clinical course, and the various available prognostic markers. In some instances, subjects previously analyzed for leukemic cell kinetics will be re-studied to determine if birth and death rates have changed. 2. Determine the kinetic profiles of normal blood B cell subsets from healthy aging subjects. The reason for this is to determine any environmental factors that are associated with aging, 50 years or older. 3. Compare the kinetic profiles of B-CLL cells with those of B cell clonal amplifications detectable in some normal aging individuals and unaffected family members who are genetically informative relatives of B-CLL patients. We are looking for genetically similar adults 18 years or older to determine factors that are associated with the development of CLL as well as environmental factors that are associated with aging. 4. Analyze the proliferating B-CLL cells, and the clonally expanded normal B cells from elderly individuals and unaffected family members who are genetically informative relatives of B-CLL patients, for the presence of cytogenetic abnormalities that differ from those of the resting B-CLL pool.