PREVENTION OF ORAL CANCER BY TEA

通过茶预防口腔癌

• 批准号: 7951989
• 负责人: FUNG-LUNG CHUNG
• 金额: $ 48.05万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7951989
• 关键词: Apoptosis; Area; Biological Assay; Black Tea; Blood; Cells; Cigarette; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA analysis; Deglutition; Double-Blind Method; Enzymes; Funding; Grant; Green tea; High Pressure Liquid Chromatography; Institution; Molecular; Oral; Placebos; Pleomorphism; Powder dose form; Protocols documentation; RNA; Randomized Controlled Clinical Trials; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Running; Smoker; Source; Tea; United States National Institutes of Health; Urine; Water; drinking; experience; keratinocyte; non-smoker; oral cancer prevention; polyphenol; prospective; protein expression; treatment duration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is R01-funded double-blind, prospective, randomized trial assessing the molecular and cellular effects of tea treatment on oral cells in smokers (> 20 cigarettes/day) and non smokers. The treatments are: green tea (400 mg of tea powder per cup), black tea, caffeinated water, and water (placebo). There is a 3-week run in period, a 4-week treatment period, and a 2-week washout period. The subjects will drink and swallow the materials in a prescribed specific manner; a total of 5 cups/day will be consumed. The PI is extremely experienced in this area. The cellular studies to be performed are varied. Oral keratinocytes will be examined for: pleomorphism, hyperchromatism, binucleation, analysis of DNA, enals and GSH, RT-PCR for enzyme expression, and protein and RNA isolation. Urine will be studied by HPLC for tea polyphenols and blood DNA will be analyzed. Apoptosis will be studied. Page 12 of the protocol indicates the studies to be performed but it is extremely cursory without a specific description of each assay, it's use, and possible interferences or problems.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这是R 01资助的双盲，前瞻性，随机试验，评估茶治疗对吸烟者（> 20支/天）和非吸烟者口腔细胞的分子和细胞影响。治疗方法是：绿色茶（每杯400毫克茶粉），红茶，含咖啡因的水和水（安慰剂）。有3周的导入期、4周的治疗期和2周的洗脱期。受试者将以规定的特定方式饮用和吞咽材料;每天总共饮用5杯。PI在这方面非常有经验。待进行的细胞研究多种多样。将检查口腔角质形成细胞的：多形性、增色、双核、DNA、烯醇和GSH分析、酶表达的RT-PCR以及蛋白质和RNA分离。将通过HPLC研究尿液中的茶多酚，并分析血液DNA。将研究细胞凋亡。方案第12页指出了要进行的研究，但非常粗略，没有对每种检测试剂盒、其用途和可能的干扰或问题进行具体描述。