UNDERSTANDING THE CHROMOSOMAL SEGREGATION ORGANIZATION IN M TUBERCULOSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议研究人类结核分枝杆菌染色体运输的细胞器--segroome的组织。细菌染色体分离过程与DNA复制有关，目前还不是很清楚。两种蛋白质(ParA和PARB)和一组原始-近端DNA序列(PARS)形成了结构。ParA是一种驱动这一过程的马达蛋白质。PARB与PARS DNA序列形成核蛋白复合体。PARB-PARS复合体可能在分离过程开始时将两条染色体配对。我们的目的是阐明PARB-PARS复合体的化学计量和三维结构。从SSRL获得的X射线溶液散射数据(SAXS)将与额外的实验数据和同源建模方法相结合，建立PARB-PARS络合物的模型。这个模型将揭示它如何在分裂和细胞分裂之前配对两条染色体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to study the organization of segrosome, the cellular apparatus for chromosome trafficking in human pathogen Mycobacterium tuberculosis. Bacterial chromosome segregation process is coupled to DNA replication and is not well-understood. Two proteins (ParA and ParB) and a set of original-proximal DNA sequences (parS) form the segrosome machinery. ParA is a motor protein that drives this process. ParB forms a nucleo-protein complex with the parS DNA sequences. The ParB-parS complex probably serves to pair-up the two chromosomes at the beginning of the segregation process. Our aim is to elucidate the stoichiometry and the three-dimensional organization of ParB-parS complex for the segrosome of M. tuberculosis. X-ray solution scattering data (SAXS) obtained from SSRL will be combined with additional experimental data and homology modeling approach to build a model of the ParB-parS complex. This model will reveal how it pairs-up the two chromosomes prior to partition and cell division.