UNDERSTANDING THE CHROMOSOMAL SEGREGATION ORGANIZATION IN M TUBERCULOSIS

了解结核分枝杆菌中的染色体分离组织

• 批准号: 8362234
• 负责人: Barnali Chaudhuri
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362234
• 关键词: Bacterial Chromosomes; Cell division; Chromosome Segregation; Chromosomes; Complex; Coupled; DNA Sequence; DNA biosynthesis; Data; Funding; Grant; Homology Modeling; Human; Modeling; Motor; Mycobacterium tuberculosis; National Center for Research Resources; Principal Investigator; Process; Proteins; Radiation; Research; Research Infrastructure; Resources; Roentgen Rays; Solutions; Source; United States National Institutes of Health; cost; data modeling; pathogen; protein complex; segregation; stoichiometry; structural biology; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We propose to study the organization of segrosome, the cellular apparatus for chromosome trafficking in human pathogen Mycobacterium tuberculosis. Bacterial chromosome segregation process is coupled to DNA replication and is not well-understood. Two proteins (ParA and ParB) and a set of original-proximal DNA sequences (parS) form the segrosome machinery. ParA is a motor protein that drives this process. ParB forms a nucleo-protein complex with the parS DNA sequences. The ParB-parS complex probably serves to pair-up the two chromosomes at the beginning of the segregation process. Our aim is to elucidate the stoichiometry and the three-dimensional organization of ParB-parS complex for the segrosome of M. tuberculosis. X-ray solution scattering data (SAXS) obtained from SSRL will be combined with additional experimental data and homology modeling approach to build a model of the ParB-parS complex. This model will reveal how it pairs-up the two chromosomes prior to partition and cell division.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们建议研究组织的segrosome，细胞器的染色体贩运在人类病原体结核分枝杆菌。细菌染色体分离过程与DNA复制相关联，但尚未得到很好的理解。两种蛋白质（帕拉和ParB）和一组原始近端DNA序列（parS）形成了分离体机制。帕拉是驱动这一过程的马达蛋白。ParB与parS DNA序列形成核蛋白复合物。ParB-parS复合体可能在分离过程开始时起着使两条染色体配对的作用。我们的目的是阐明ParB-parS复合物在M.结核从SSRL获得的X射线溶液散射数据（SAXS）将与额外的实验数据和同源建模方法相结合，以建立ParB-parS复合物的模型。该模型将揭示它如何在分割和细胞分裂之前配对两条染色体。