UNDERSTANDING THE CHROMOSOMAL SEGROSOME ORGANIZATION IN MYCOBACTERIUM TUBERCULOS

了解结核分枝杆菌中的染色体区段结构

• 批准号: 8170327
• 负责人: Barnali Chaudhuri
• 金额: $ 0.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170327
• 关键词: Chromosome Segregation; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Sequence; Data; Filament; Funding; Genus Mycobacterium; Grant; Homology Modeling; Human; Institution; Modeling; Motor; Mycobacterium tuberculosis; Nucleoproteins; Process; Proteins; Research; Research Personnel; Resources; Roentgen Rays; Solutions; Source; United States National Institutes of Health; pathogen; protein complex; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to study the organization of segrosome, the cellular apparatus for chromosome segregation in human pathogen Mycobacterium tuberculosis. Two proteins (ParA and ParB) and a set of original-proximal DNA sequences (parS) form the segrosome machinery. ParA is a motor protein that drives this process. ParB forms a nucleo-protein complex with the parS DNA sequence and polymerize on the parS-proximal DNA scaffold to form a superstructure. Our aim is to elucidate the three-dimensional organization of this nucleoprotein complex for the segrosome of M. tuberculosis. X-ray solution scattering data (SAXS) obtained from SSRL will be combined with additional experimental data and homology modeling approach to build a model of the ParB-parS complex and the nucleoprotein filament.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们建议研究组织的segrosome，细胞器的染色体分离在人类病原体结核分枝杆菌。两种蛋白质（帕拉和ParB）和一组原始近端DNA序列（parS）形成了分离体机制。帕拉是驱动这一过程的马达蛋白。ParB与parS DNA序列形成核蛋白复合物，并粘附在parS近端DNA支架上以形成超结构。我们的目的是阐明M.结核从SSRL获得的X射线溶液散射数据（SAXS）将与额外的实验数据和同源建模方法相结合，以建立ParB-parS复合物和核蛋白丝的模型。