UNDERSTANDING THE CHROMOSOMAL SEGROSOME ORGANIZATION IN MYCOBACTERIUM TUBERCULOS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议研究人类结核分枝杆菌染色体分离的细胞器--segroome的组织。两种蛋白质(ParA和PARB)和一组原始-近端DNA序列(PARS)形成了结构。ParA是一种驱动这一过程的马达蛋白质。PARB与PARS DNA序列形成核蛋白复合体，并在PARB近端DNA支架上聚合形成超结构。我们的目的是阐明结核分枝杆菌菌落的核蛋白复合体的三维结构。从SSRL获得的X射线溶液散射数据(SAXS)将与额外的实验数据和同源建模方法相结合，建立PARB-PARS复合体和核蛋白细丝的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to study the organization of segrosome, the cellular apparatus for chromosome segregation in human pathogen Mycobacterium tuberculosis. Two proteins (ParA and ParB) and a set of original-proximal DNA sequences (parS) form the segrosome machinery. ParA is a motor protein that drives this process. ParB forms a nucleo-protein complex with the parS DNA sequence and polymerize on the parS-proximal DNA scaffold to form a superstructure. Our aim is to elucidate the three-dimensional organization of this nucleoprotein complex for the segrosome of M. tuberculosis. X-ray solution scattering data (SAXS) obtained from SSRL will be combined with additional experimental data and homology modeling approach to build a model of the ParB-parS complex and the nucleoprotein filament.