ROTAVIRUS AND NORWALK VIRUS

轮状病毒和诺沃克病毒

• 批准号: 7953755
• 负责人: Bidadi Venkataram Prasad
• 金额: $ 1.74万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953755
• 关键词: Acute; Architecture; BCAR3 gene; Caliber; Capsid; Complex; Computer Retrieval of Information on Scientific Projects Database; Double-Stranded RNA; Electron Microscopy; Epidemic; Funding; Gastroenteritis; Genetic Transcription; Genome; Grant; Institution; Molecular; Nonstructural Protein; Norovirus; Norwalk virus; Process; Research; Research Personnel; Resolution; Resources; Rotavirus; Site; Source; Structure; United States National Institutes of Health; Viral; Virus; Virus Diseases; base; fascinate; infancy; macromolecule; particle; pathogen; prototype

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rotavirus is a major pathogen of infantile gastroenteritis. It is an icosahedral virus, 1000 ¿ in diameter, with a complex architecture consisting of three concentric capsid layers enclosing 11 dsRNA segments. Over several years we have been trying to determine the structure of various forms of this medically important virus. We are presently using the NCMI facility to carry out high-resolution structural analysis of transcriptionally competent, transcriptionally incompetent and transcriptionally active forms of rotavirus particles, to understand molecular mechanisms underlying the fascinating process of endogenous transcription in these viruses. In addition, we also study various molecular components of rotavirus. Rotavirus viroplasms form by expression of nonstructural proteins NSP2 and NSP5. These viroplasms formed during viral infection provide excusive sites for genome replication/packaging and capsid assembly. Little is known about the structural basis of how these processes are coordinated within these viral factories. Norwalk virus is a prototype norovirus, which are causative agents of acute epidemic gastroenteritis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 轮状病毒是小儿胃肠炎的主要病原体。 它是一种二十面体病毒，直径为 1000 Å，具有复杂的结构，由包围 11 个 dsRNA 片段的三个同心衣壳层组成。多年来，我们一直试图确定这种医学上重要的病毒的各种形式的结构。 我们目前正在使用 NCMI 设施对轮状病毒颗粒的转录能力、转录能力和转录活性形式进行高分辨率结构分析，以了解这些病毒中令人着迷的内源转录过程背后的分子机制。 此外，我们还研究轮状病毒的各种分子成分。 轮状病毒病毒浆由非结构蛋白 NSP2 和 NSP5 的表达形成。 这些病毒感染过程中形成的病毒质为基因组复制/包装和衣壳组装提供了专用位点。 人们对这些过程在这些病毒工厂内如何协调的结构基础知之甚少。 诺瓦克病毒是诺如病毒的原型，是急性流行性胃肠炎的病原体。