ROTAVIRUS AND NORWALK VIRUS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 轮状病毒是婴幼儿胃肠炎的主要病原体。它是一种二十面体病毒，直径1000？，具有由三个同心衣壳层组成的复杂结构，包裹着11个dsRNA片段。几年来，我们一直试图确定这种具有重要医学意义的病毒的各种形式的结构。我们目前正在使用NCMI设施对轮状病毒颗粒的转录活性、转录不活性和转录活性形式进行高分辨率结构分析，以了解这些病毒内源转录迷人过程的分子机制。 此外，我们还研究了轮状病毒的各种分子成分。 轮状病毒由非结构蛋白NSP2和NSP5的表达形成。这些在病毒感染过程中形成的病毒质为基因组复制/包装和衣壳组装提供了执行部位。关于这些过程如何在这些病毒工厂内协调的结构基础，人们知之甚少。 诺沃克病毒是诺沃克病毒的原型，是急性流行性胃肠炎的病原体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rotavirus is a major pathogen of infantile gastroenteritis. It is an icosahedral virus, 1000 ¿ in diameter, with a complex architecture consisting of three concentric capsid layers enclosing 11 dsRNA segments. Over several years we have been trying to determine the structure of various forms of this medically important virus. We are presently using the NCMI facility to carry out high-resolution structural analysis of transcriptionally competent, transcriptionally incompetent and transcriptionally active forms of rotavirus particles, to understand molecular mechanisms underlying the fascinating process of endogenous transcription in these viruses. In addition, we also study various molecular components of rotavirus. Rotavirus viroplasms form by expression of nonstructural proteins NSP2 and NSP5. These viroplasms formed during viral infection provide excusive sites for genome replication/packaging and capsid assembly. Little is known about the structural basis of how these processes are coordinated within these viral factories. Norwalk virus is a prototype norovirus, which are causative agents of acute epidemic gastroenteritis.