Microscopy and Flow Cytometry

项目总结 显微镜核心和流式细胞术(核心B)将提供与标准相关的专业知识和服务 电子显微镜(EM)，以及荧光显微镜和流式细胞术，根据每个需要 三个项目组件。这种用于标本制备和成像的集中式设施将耗资- 有效和便利更有序和高效地使用EM和荧光显微镜资源 及时完成拟建项目。要求的预算是适度的，年薪为50% 显微镜/成像实验室经理，以及EM的研究技术员和50%的服务合同 还有荧光显微镜。对于流式细胞仪专家来说，新增加的功能是24%的时间。的具体功能 核心将包括：(1)用于电子显微镜的标本制备；(2)重组病毒的成像 颗粒及其与碳水化合物和抗体的复合体，重组脂泡以评价纯度 和浓度；(3)杯状病毒感染动物和人类粪便样本的成像；(4)对 病毒感染细胞的染色薄片；(5)免疫电子显微镜；(6)数据获取、共享和 存档；(7)计划和日常EM维护；(8)荧光样品准备 显微镜；(9)流式细胞术，以获得病毒感染细胞和从 诺沃克病毒感染者(10)显微镜和流量的日程安排和日常维护 细胞仪。核心主任和副主任将协调显微镜检查和流程的所有方面 细胞学核心。

PROJECT SUMMARY The Microscopy Core and Flow Cytometry (Core B) will provide expertise and service related to standard electron microscopy (EM), as well as fluorescent microscopy and flow cytometry as needed by each of the three project components. Such a centralized facility for specimen preparation and imaging will be cost- effective and facilitate more orderly and efficient use of the EM and fluorescent microscope resources to complete the proposed projects in a timely fashion. The requested budget is modest with a 50% salary for a microscopy/imaging lab manager and as well as a research technician and 50% service contract for the EM and fluorescent microscopes. A new addition is 24% time for a flow cytometry expert. The specific functions of the core will include: (1) specimen preparation for electron microscopy; (2) imaging of viruses, recombinant particles, and their complexes with –carbohydrate and antibodies, reconstituted lipid vesicles to evaluate purity and concentration; (3) imaging of fecal samples from calicivirus infected animals and humans; (4) imaging of stained thin sections of virus infected cells; (5) immuno-electron microscopy; (6) data acquisition, sharing, and archiving; (7) scheduling and day-to-day EM maintenance; (8) specimen preparation for fluorescent microscopy; (9) flow cytometry to obtain quantitative data on virus-infected cells and on PBMCs collected from norovirus-infected individuals (10) scheduling and day-to-day maintenance of the microscopes and flow cytometers. The Core Directors and Associate Director will coordinate all aspects of the Microscopy and Flow Cytometry Core.