Microscopy

显微镜检查

• 批准号: 7774783
• 负责人: Bidadi Venkataram Prasad
• 金额: $ 17.27万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7774783
• 关键词: Animals; Archives; Binding; Budgets; Calicivirus; Carbohydrates; Cells; Complex; Computers; Data Analyses; Data Collection; Electron Microscope; Electron Microscopy; Fluorescence Microscopy; Gold; Grant; Human; Image; Immune; Label; Maintenance; Microscope; Microscopy; Microtomy; Preparation; Research; Research Personnel; Resources; Role; Sampling; Schedule; Specimen; Staining method; Stains; Training; Virion; Virus; base; cost; data acquisition; fluorescence microscope; operation; particle; programs; recombinant virus

The role ofthe Microscopy Core is to provide a centralized facility to serve the electron microscopy and fluorescence microscopy needs of all ofthe investigators in the Program Project Proposal. This integrated facility is cost-effective and facilitates more orderly and efficient use of microscopy resources to complete the proposed projects in a timely fashion. A modest budget is requested, based on the use of this facility during the current grant period, to enable proper maintenance and smooth operation of the facility. The specific functions ofthe Core, as required routinely by the three projects, will include: (1) Specimen preparation for electron microscopy (2) EM imaging of viruses, recombinant particles, and virus-carbohydrate complexes to evaluate purity, particle integrity, and concentration (3) EM imaging of fecal samples from calicivirus-infected animals and humans (4) EM imaging of stained and fixed thin sections of virus-infected cells to assess cell binding (5) Immune electron microscopy using gold labels (6) Data acquisition, sharing, and archiving from the electron microscope (7) Scheduling and day-to-day EM maintenance (8) Specimen preparation for fluorescent, confocal and deconvolution microscopy (9) Scheduling and day-to-day maintenance of the fluorescent, confocal and deconvolution microscopes 10) Data acquisition and interpretation using fluorescent, confocal and deconvolution microscopes

显微镜核心的作用是提供集中式设施，以服务电子显微镜和 计划项目建议中所有研究人员的荧光显微镜需求。这整合了 设施具有成本效益，并促进了更有序，更有效地利用显微镜资源来完成 及时提议的项目。根据在此期间使用此设施的使用，要求提供适度的预算 当前的赠款期，以实现设施的适当维护和平稳运行。 根据三个项目通常要求，核心的特定功能将包括： （1）电子显微镜的样品制备 （2）病毒，重组颗粒和病毒碳水化合物复合物的EM成像，以评估纯度， 粒子完整性和浓度 （3）来自热门病毒感染动物和人类的粪便样品的成像 （4）对感染病毒感染细胞的染色和固定细胞的EM成像，以评估细胞结合 （5）使用金标签的免疫电子显微镜 （6）电子显微镜的数据获取，共享和存档 （7）安排和日常EM维护 （8）用于荧光，共聚焦和反卷积显微镜的样品制备 （9）荧光，共聚焦和反卷积显微镜的调度和日常维护 10）使用荧光，共焦和反卷积显微镜的数据采集和解释