Microscopy

显微镜检查

• 批准号: 7774783
• 负责人: Bidadi Venkataram Prasad
• 金额: $ 17.27万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7774783
• 关键词: Animals; Archives; Binding; Budgets; Calicivirus; Carbohydrates; Cells; Complex; Computers; Data Analyses; Data Collection; Electron Microscope; Electron Microscopy; Fluorescence Microscopy; Gold; Grant; Human; Image; Immune; Label; Maintenance; Microscope; Microscopy; Microtomy; Preparation; Research; Research Personnel; Resources; Role; Sampling; Schedule; Specimen; Staining method; Stains; Training; Virion; Virus; base; cost; data acquisition; fluorescence microscope; operation; particle; programs; recombinant virus

The role ofthe Microscopy Core is to provide a centralized facility to serve the electron microscopy and fluorescence microscopy needs of all ofthe investigators in the Program Project Proposal. This integrated facility is cost-effective and facilitates more orderly and efficient use of microscopy resources to complete the proposed projects in a timely fashion. A modest budget is requested, based on the use of this facility during the current grant period, to enable proper maintenance and smooth operation of the facility. The specific functions ofthe Core, as required routinely by the three projects, will include: (1) Specimen preparation for electron microscopy (2) EM imaging of viruses, recombinant particles, and virus-carbohydrate complexes to evaluate purity, particle integrity, and concentration (3) EM imaging of fecal samples from calicivirus-infected animals and humans (4) EM imaging of stained and fixed thin sections of virus-infected cells to assess cell binding (5) Immune electron microscopy using gold labels (6) Data acquisition, sharing, and archiving from the electron microscope (7) Scheduling and day-to-day EM maintenance (8) Specimen preparation for fluorescent, confocal and deconvolution microscopy (9) Scheduling and day-to-day maintenance of the fluorescent, confocal and deconvolution microscopes 10) Data acquisition and interpretation using fluorescent, confocal and deconvolution microscopes

显微镜核心的作用是提供一个集中的设施，以服务于电子显微镜， 荧光显微镜的需要，所有ofthe研究人员在计划项目建议书。这一综合 该设施具有成本效益，便于更有序和有效地使用显微镜资源，以完成 及时提出项目。根据该设施在2005年期间的使用情况， 以确保设施得到妥善维修及运作畅顺。 按照这三个项目的例行要求，核心小组的具体职能将包括： (1)电子显微镜样品制备 (2)病毒、重组颗粒和病毒-碳水化合物复合物的EM成像，以评价纯度， 颗粒完整性和浓度 (3)杯状病毒感染动物和人类粪便样本的EM成像 (4)病毒感染细胞的染色和固定薄切片的EM成像，以评估细胞结合 (5)金标记免疫电镜 (6)电子显微镜的数据采集、共享和存档 (7)调度和日常EM维护 (8)用于荧光、共聚焦和去卷积显微镜的样品制备 (9)荧光显微镜、共聚焦显微镜和去卷积显微镜的安排和日常维护 10)使用荧光、共聚焦和去卷积显微镜进行数据采集和解释