TRYPANOSOMA FLAGELLUM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 布鲁氏鞭毛是一种多组分、多功能的细胞器，在致病过程中起着至关重要的作用。该鞭毛不仅含有经典的“9+2”轴丝，还含有一条结晶性的束旁杆(PFR)。该项目的生物学兴趣是PFR的两个主要结构成分的结构组织，这两个主要结构成分分别是69KD和73KD的PFR1和PFR2。通过序列分析，它们似乎主要由卷曲的线圈基序组成。通过RNA干扰耗尽一个PFR蛋白并不影响寄生虫的生长或轴丝的分裂或形成。然而，它完全取消了PFR的组装，导致细胞运动性和感染性的缺陷。我们计划用冷冻电子显微镜来研究这种复杂细胞器的结构组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The T. brucei flagellum is a multi-component, multifunctional organelle critical for pathogenesis. This flagellum contains not only the classic "9+2" axoneme but also a crystalline paraflagellar rod (PFR). The biological interest in this project is the structural organization of the two major structural components of PFR which have been characterized, PFR1 and PFR2 at 69KD and 73KD, respectively. They appear by sequence analysis to consist largely of coiled-coil motifs. Depletion of one PFR protein by RNA interference did not affect parasite growth or division nor formation of the axoneme. However, it completely abolished the assembly of the PFR, resulting in defects in cell motility and infectivity. We plan to use cryo-ET to study the structural organization of this complex organelle.