TRYPANOSOMA FLAGELLUM

鞭毛锥虫

• 批准号: 8361097
• 负责人: Cynthia He
• 金额: $ 7.36万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361097
• 关键词: Affect; Biological; Complex; Defect; Electron Microscopy; Flagella; Funding; Grant; Growth; National Center for Research Resources; Organelles; Parasites; Pathogenesis; Principal Investigator; Proteins; RNA Interference; Research; Research Infrastructure; Resources; Sequence Analysis; Source; Trypanosoma brucei brucei; United States National Institutes of Health; cell motility; cost; interest; macromolecule; retinal rods

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The T. brucei flagellum is a multi-component, multifunctional organelle critical for pathogenesis. This flagellum contains not only the classic "9+2" axoneme but also a crystalline paraflagellar rod (PFR). The biological interest in this project is the structural organization of the two major structural components of PFR which have been characterized, PFR1 and PFR2 at 69KD and 73KD, respectively. They appear by sequence analysis to consist largely of coiled-coil motifs. Depletion of one PFR protein by RNA interference did not affect parasite growth or division nor formation of the axoneme. However, it completely abolished the assembly of the PFR, resulting in defects in cell motility and infectivity. We plan to use cryo-ET to study the structural organization of this complex organelle.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 布鲁氏链球菌鞭毛是一种多功能的多功能细胞器，对发病机理至关重要。该鞭毛不仅包含经典的“ 9+2”轴突，还包含晶体板岩杆（PFR）。该项目的生物学兴趣是PFR的两个主要结构组成部分的结构组织，分别在69kD和73kD时表征了PFR1和PFR2。它们通过序列分析出现，主要由盘绕螺旋序列组成。 RNA干扰对一种PFR蛋白的耗竭不会影响寄生虫的生长或分裂或轴突形成。但是，它完全消除了PFR的组装，从而导致细胞运动和感染性缺陷。我们计划使用Cryo-Et研究该复杂细胞器的结构组织。