ULTRA-MICRO OXYGEN SENSOR DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该资源见证了对针对新陈代谢的应用程序的日益增长的需求。为了促进这一点，我们开发了几种新技术或整合了其他技术，以允许通过几种方法研究细胞新陈代谢。其中一些在其他地方也有报道。一项关键技术是应用了自参照微型传感器，到目前为止，这种传感器都是手工建造的(见SPID 0047)，结果各不相同。虽然我们在这里的效率有了很大的提高，但手工生产安培传感器仍然是一个瓶颈。为了避免这一点，我们需要在建筑中加入较少的可变性，更好地定义我们的最佳条件，并最终纳入某种形式的自动化或半自动化生产。总体而言，我们需要能够提供满足信号检测的非侵入性调制技术的严格要求的微电极。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The resource has witnessed an increasing demand for applications targeting metabolism. To facilitate this we have developed several new technologies or integrated others to allow the study of cellular metabolism through several approaches. A number of these are reported on elsewhere. A key technique has been the application of self-referencing microsensors which to date have been constructed by hand (see SPID 0047) and with variable results. Although our efficiency here has greatly improved, manual production of amperometric sensors remains a bottleneck. To circumvent this we need to incorporate less variability in the construction, better define our optimal conditions and ultimately incorporate some form of automated or semi automated production. Overall we need to be in the position to supply microelectrodes meeting the exacting requirements of a non-invasive modulation technique for signal detection.