DEVELOPING RAPID RESPONSE ION SELECTIVE ELECTRODES

开发快速响应离子选择电极

• 批准号: 7953840
• 负责人: PETER JS SMITH
• 金额: $ 3.36万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953840
• 关键词: Cells; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Data; Diffusion; Environment; Extracellular Space; Funding; Grant; Institution; Ion Channel; Ion-Selective Electrodes; Ions; Noise; Process; Reaction Time; Research; Research Personnel; Resources; Signal Transduction; Source; Testing; Time; United States National Institutes of Health; density; extracellular; improved; response; three-dimensional modeling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a subproposal within one of the core aims of the Biocurrents Research Center. We are currently developing ion-selective electrodes (ISEs) with improved response time (<1ms) and signal-to-noise ratio in order to extract meaningful single ion channel characteristics from global extracellular ionic gradients. These characteristics include mean open time, conductance and channel density. Prior to collecting cellular data and testing the above hypothesis, it is necessary to determine the influence of diffusion processes on ion fluxes at a finite distance from the point of efflux or influx. Therefore, we have set out to model the 3D environment of the boundary layer of the cell and demonstrate whether the ISE can reliably resolve unitary ion currents from clusters of ion channels within the extracellular space.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这是生物流量研究中心核心目标之一的一个子提案。我们目前正在开发具有更好的响应时间(1ms)和信噪比的离子选择电极(ISE)，以便从全球细胞外离子梯度中提取有意义的单离子通道特征。这些特性包括平均打开时间、电导和通道密度。在收集细胞数据和验证上述假设之前，有必要确定扩散过程对离流出点或流入点有限距离处的离子通量的影响。因此，我们已经着手对细胞边界层的3D环境进行建模，并论证ISE是否能够可靠地从细胞外空间内的离子通道簇中解析出单一的离子电流。