TOMOGRAPHIC PHASE MICROSCOPY

断层相显微镜

• 批准号: 7955860
• 负责人: WOO-BAEG CHOI
• 金额: $ 3.8万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955860
• 关键词: 3-Dimensional; Algorithms; Back; Biological; Biomedical Research; Cells; Collection; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Image; Institution; Lasers; Life; Lighting; Maps; Microscopy; Optical Methods; Phase; Refractive Indices; Research; Research Personnel; Resolution; Resources; Sampling; Source; Techniques; Tissues; United States National Institutes of Health; disease diagnosis; heterodyning; light scattering; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed a technique for quantitative three-dimensional mapping of refractive index in live biological cells and tissues. The projection of sample refractive index is imaged using a Mach-Zehnder phase-shifting heterodyne interferometer with multiple directions of illumination. A 3-D tomographic reconstruction is then derived from the collection of 2-D projection phase images using a filtered back-projection and iterative constraint algorithm. Refractive index maps will have applications in high resolution microscopy, cellular light scattering, and optical methods for disease diagnosis and imaging.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们开发了一种在活的生物细胞和组织中定量绘制折射率三维图谱的技术。利用马赫-曾德尔相移外差干涉仪对样品的折射率投影进行多方向照明成像。然后，使用滤波反投影和迭代约束算法，从二维投影相位图像的集合中获得三维层析重建。折射率图将在高分辨率显微镜、细胞光散射以及疾病诊断和成像的光学方法中得到应用。