OPTICAL DIFFRACTION TOMOGRAPHY FOR HIGH RESOLUTION 3D LIVE CELL IMAGING

用于高分辨率 3D 活细胞成像的光学衍射断层扫描

• 批准号: 7955867
• 负责人: WOO-BAEG CHOI
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955867
• 关键词: Algorithms; Biomedical Research; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Image; Individual; Institution; Lasers; Life; Light; Measures; Microscopy; Nature; Optics; Organelles; Phase; Property; Refractive Indices; Research; Research Personnel; Resolution; Resources; Sampling; Source; Structure; System; Techniques; Thick; Three-Dimensional Image; United States National Institutes of Health; base; cellular imaging; reconstruction; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recently, we demonstrated tomographic phase microscopy capable of measuring refractive index of a live cell with high resolution. Three dimensional imaging of a live cell provides a way to investigate optical properties of individual organelles. In this project, we have developed new algorithm based on the diffractive nature of light. With the help of new algorithm, we can clearly resolve detailed structures in the out-of focus plane which has not been resolved with current tomography technique. This algorithm makes it possible to obtain high resolution 3D images for a much thicker sample than the depth of focus of imaging system. We are in pursuit of applying this new algorithm for 3D reconstruction from the reflected light.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 最近，我们证明了层析相位显微镜能够测量高分辨率的活细胞的折射率。活细胞的三维成像提供了一种研究单个细胞器的光学特性的方法。在这个项目中，我们已经开发了新的算法的基础上衍射性质的光。新算法可以清晰地分辨出现有层析成像技术无法分辨的离焦面内的细节结构。该算法使得对于比成像系统的焦深厚得多的样品获得高分辨率的3D图像成为可能。我们正在寻求将这种新算法应用于从反射光进行3D重建。