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OPTICAL DIFFRACTION TOMOGRAPHY FOR HIGH RESOLUTION 3D LIVE CELL IMAGING

OPTICAL DIFFRACTION TOMOGRAPHY FOR HIGH RESOLUTION 3D LIVE CELL IMAGING
用于高分辨率 3D 活细胞成像的光学衍射断层扫描
批准号:
7955867
负责人:
WOO-BAEG CHOI
金额:
$1.9万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-06-01 至 2010-05-31

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中文摘要
翻译
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recently, we demonstrated tomographic phase microscopy capable of measuring refractive index of a live cell with high resolution. Three dimensional imaging of a live cell provides a way to investigate optical properties of individual organelles. In this project, we have developed new algorithm based on the diffractive nature of light. With the help of new algorithm, we can clearly resolve detailed structures in the out-of focus plane which has not been resolved with current tomography technique. This algorithm makes it possible to obtain high resolution 3D images for a much thicker sample than the depth of focus of imaging system. We are in pursuit of applying this new algorithm for 3D reconstruction from the reflected light.
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会议论文
MEASURING CELL REFRACTIVE INDEX BY CONFOCAL REFLECTANCE AND HP MICROSCOPY
DEVELOPMENT OF PARALLEL COMPUTATION SYSTEM FOR TOMOGRAPHIC RECONSTRUCTION
DEVELOPMENT OF PARALLEL COMPUTATION SYSTEM FOR TOMOGRAPHIC RECONSTRUCTION
MEASURING CELL REFRACTIVE INDEX BY CONFOCAL REFLECTANCE AND HP MICROSCOPY
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