OPTICAL DIFFRACTION TOMOGRAPHY FOR HIGH RESOLUTION 3D LIVE CELL IMAGING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近，我们展示了断层位相显微镜能够以高分辨率测量活细胞的折射率。活细胞的三维成像为研究单个细胞器的光学性质提供了一种方法。在这个项目中，我们开发了基于光的绕射性质的新算法。在新算法的帮助下，我们可以清楚地分辨出当前层析技术无法解决的焦外平面内的细节结构。该算法可以在比成像系统焦深大得多的样品上获得高分辨率的3D图像。我们正在追求将这种新的算法应用于从反射光进行三维重建。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recently, we demonstrated tomographic phase microscopy capable of measuring refractive index of a live cell with high resolution. Three dimensional imaging of a live cell provides a way to investigate optical properties of individual organelles. In this project, we have developed new algorithm based on the diffractive nature of light. With the help of new algorithm, we can clearly resolve detailed structures in the out-of focus plane which has not been resolved with current tomography technique. This algorithm makes it possible to obtain high resolution 3D images for a much thicker sample than the depth of focus of imaging system. We are in pursuit of applying this new algorithm for 3D reconstruction from the reflected light.