MEASURING CELL REFRACTIVE INDEX BY CONFOCAL REFLECTANCE AND HP MICROSCOPY

通过共焦反射和 HP 显微镜测量细胞折射率

• 批准号: 7955870
• 负责人: WOO-BAEG CHOI
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955870
• 关键词: Biological Assay; Biomedical Research; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; In Situ; Institution; Label; Lasers; Life; Light; Measurement; Measures; Microscope; Microscopy; Optics; Phase; Proteins; Refractive Indices; Research; Research Personnel; Resolution; Resources; Source; Techniques; United States National Institutes of Health; Work; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Quantitative phase measurements have achieved uniquely stable and high resolution measurements of phase and it becomes the appealing feature that they can be interfaced with an existing optical microscope for biomedical research. This label-free quantitative phase measurement allows us to study live cell without perturbation. However the technique offers only a total phase that is the line integral of the refractive index and the light beam's path through a cell. This work demonstrates a combining confocal reflectance and quantitative phase microscopy can be used to better estimate an average refractive index of living cell. Using the confocal reflectance to obtain the cell contour and the Hilbert phase microscopy to obtain the phase shift induced by the cell, the average refractive index of cell which presents protein contents (dry-mass) can be determined without postulating or constraining the cell to a known geometry. This approach can provide novel biological assays of label-free living cells in situ.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 定量相位测量已经实现了独特的稳定性和高分辨率的相位测量，它成为有吸引力的功能，它们可以与现有的光学显微镜的生物医学研究。这种无标记的定量相位测量使我们能够在不受干扰的情况下研究活细胞。然而，该技术仅提供总相位，其是折射率和光束通过细胞的路径的线积分。 这项工作表明，结合共焦反射和定量相位显微镜可以用来更好地估计活细胞的平均折射率。利用共焦反射率获得细胞轮廓，利用希尔伯特相位显微镜获得细胞诱导的相移，可以在不假设或限制细胞几何形状的情况下，确定呈现蛋白质含量（干质量）的细胞的平均折射率。这种方法可以提供新的无标记活细胞原位生物测定。