IDENTIFYING CONDENSIN INTERACTING PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 在每次细胞分裂过程中，基因组必须准确地复制并将一套完整的染色体分离到每个子细胞中。染色体分离的失败对人类健康有严重的后果，因为产生具有不适当染色体数目的细胞可能导致细胞死亡，出生缺陷或癌症。我们的研究重点是凝聚素，保守的蛋白质，确保准确的染色体分离，通过组织染色体结构在减数分裂和有丝分裂。凝聚素结合染色体，并被认为利用ATP水解的能量将DNA缠绕成大环。我们证明了C. elegans有两个缩合素复合物，它们具有重叠的组分，并且在有丝分裂和基因调控中具有不同的功能。我们以前的工作也表明，凝聚素的功能超出了简单的压缩，以促进姐妹染色单体的决议和着丝粒组织。为了了解更多关于凝聚素的信息，我们现在正在采用功能蛋白质组学方法来鉴定凝聚素相互作用蛋白。初步的结果已经确定了新的凝聚素亚基，意想不到的亚基相互作用，和调节蛋白，指向绑定凝聚素功能的细胞周期的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.