IDENTIFYING CONDENSIN INTERACTING PROTEINS

鉴定凝缩蛋白相互作用蛋白

• 批准号: 8365868
• 负责人: KIRSTEN A HAGSTROM
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365868
• 关键词: ATP Hydrolysis; Binding; Biology; Caenorhabditis elegans; Cell Cycle; Cell Death; Cell division; Cells; Centromere; Chromosome Segregation; Chromosome Structures; Chromosomes; Complex; Congenital Abnormality; DNA; Ensure; Environmental Wind; Failure; Funding; Fungal Genome; Gene Expression Regulation; Genome; Grant; Health; Human; Lead; Learning; Malignant Neoplasms; Meiosis; Mitosis; National Center for Research Resources; Principal Investigator; Proteins; Proteomics; Research; Research Infrastructure; Resolution; Resources; Sister Chromatid; Source; United States National Institutes of Health; Work; condensin; cost; daughter cell; genetic regulatory protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在每一次细胞分裂中，基因组必须准确地复制并将一套完整的染色体分离到每个子细胞中。染色体分离的失败会对人类健康造成严重后果，因为产生染色体数量不适当的细胞可能会导致细胞死亡、出生缺陷或癌症。我们的研究重点是凝集素，这是一种保守的蛋白质，通过在减数分裂和有丝分裂过程中组织染色体结构来确保准确的染色体分离。凝聚素与染色体结合，被认为利用ATP水解的能量将DNA卷曲成大环。我们发现线虫有两个具有重叠成分的凝集素复合体，它们在有丝分裂和基因调控中具有不同的功能。我们以前的工作也表明，凝聚素的功能不仅仅是简单的紧凑，而是促进姐妹染色单体的分辨和着丝粒的组织。为了更多地了解凝聚素，我们现在正在采用功能蛋白质组学的方法来鉴定凝聚素相互作用的蛋白质。初步结果已经确定了新的凝聚素亚基，意想不到的亚基相互作用，以及指向将凝聚素功能与细胞周期联系在一起的调节蛋白。