IDENTIFYING CONDENSIN INTERACTING PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在每一次细胞分裂过程中，基因组必须准确地复制和分离一个完整的 每一个子细胞中的一组染色体。染色体分离的失败有 对人类健康的严重后果，因为创造了不适当的细胞 染色体数目可能导致细胞死亡、出生缺陷或癌症。我们的研究重点是 凝集素是一种保守的蛋白质，通过组织确保准确的染色体分离 减数分裂和有丝分裂过程中的染色体结构。凝聚素与染色体结合在一起 被认为是利用三磷酸腺苷水解的能量将DNA缠绕成大环。我们证明了C. Elegans有两个凝集素复合体，它们的成分重叠，功能不同 有丝分裂与基因调控。我们之前的工作还表明，凝聚素具有 除了简单的压实外，还可以提高姐妹染色单体的分辨率和着丝粒 组织。为了更多地了解凝聚素，我们现在进行功能蛋白质组学 鉴定凝聚素相互作用蛋白的方法。初步结果确定了新的 凝集素亚基，意想不到的亚基相互作用，以及指向 将凝聚素功能与细胞周期联系在一起的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.