IDENTIFYING CONDENSIN INTERACTING PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在每一次细胞分裂中，基因组必须准确地复制并分离出完整的 一组染色体进入每个子细胞。染色体分离的失败 对人类健康的严重后果，因为创造细胞与不适当的 染色体数目会导致细胞死亡、出生缺陷或癌症。我们的研究重点是 凝聚素，保守的蛋白质，确保准确的染色体分离， 减数分裂和有丝分裂时的染色体结构。凝聚素结合染色体， 被认为是利用ATP水解的能量将DNA缠绕成大环。我们证明了C. 线虫有两个缩合素复合物，它们具有重叠的组分和不同的功能， 有丝分裂和基因调控。我们以前工作也表明， 除了简单的压实，以促进姐妹染色单体的分辨率和着丝粒 organization.为了了解更多关于凝聚素的信息，我们现在正在进行功能蛋白质组学研究， 方法来识别缩合蛋白相互作用蛋白。初步结果显示， 凝聚素亚基，意想不到的亚基相互作用，和调节蛋白，指出， 将凝聚素功能与细胞周期联系起来的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During every cell division, the genome must accurately replicate and segregate a complete set of chromosomes into each daughter cell. Failures in chromosome segregation have severe consequences for human health, since creating cells with an inappropriate chromosome number can lead to cell death, birth defects, or cancer. Our research focuses on condensins, conserved proteins that ensure accurate chromosome segregation by organizing chromosome structure during meiosis and mitosis. Condensins bind chromosomes and are thought to use the energy of ATP hydrolysis to wind DNA into large loops. We showed that C. elegans has two condensin complexes with overlapping components and distinct functions in mitosis and gene regulation. Our previous work also suggested that condensin functions beyond simple compaction to promote sister chromatid resolution and centromere organization. To learn more about condensins, we are now taking a functional proteomics approach to identify condensin-interacting proteins. Preliminary results have identified new condensin subunits, unexpected subunit interactions, and regulatory proteins that point to mechanisms for tying condensin function to the cell cycle.