GENOME-WIDE DNASE I FOOTPRINTING OF YEAST CHROMATIN
GENOME-WIDE DNASE I FOOTPRINTING OF YEAST CHROMATIN
批准号:
7957774
负责人:
STANLEY FIELDS
金额:
$1.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-09-01 至 2010-08-31
关键词:
ArchitectureBindingBinding ProteinsBinding SitesBiological AssayBiologyChromatinChromosomesComplementComputer Retrieval of Information on Scientific Projects DatabaseDNA SequenceDNA biosynthesisDNA-Binding ProteinsDNase-I FootprintingDeoxyribonuclease IDigestionFundingFungal GenomeGene ExpressionGenesGenomeGrantInstitutionOrganismProcessReadingResearchResearch PersonnelResourcesSaccharomyces cerevisiaeSiteSourceUnited States National Institutes of HealthYeastsbasechromatin immunoprecipitationgenome-widenucleaseprogramstranscription factor
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The complement of DNA-binding proteins and their occupancy of sites throughout the genome determine an organism's programs of gene expression, DNA replication and other chromosome-based processes. A detailed picture of factor binding on a genome-wide basis exists for Saccharomyces cerevisiae, obtained by a combination of transcriptional profiles, chromatin immunoprecipitation of more than 200 transcription factors, computational analyses and other assays. In an alternative approach, we have used digestion of chromatin by DNase I followed by high throughput DNA sequencing to identify sites of increased nuclease accessibility throughout the yeast genome. The resulting set of more than 10 million sequence reads provides both a global view of chromatin architecture as well as a gene-by-gene view of regulatory sequences protected from digestion by the presence of bound proteins. Unlike the case with results from chromatin immunoprecipitation, these gene-by-gene DNase I footprints can be used to directly identify transcription factor binding sites, and thereby infer their motifs. We found previously unknown binding sites in the genome for well-characterized factors, and observed other annotated binding sites that appear not to be protected from nuclease digestion under our conditions.
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依托单位:
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资助金额:$2.18万
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财政年份:2011
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负责人:STANLEY FIELDS
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依托单位:
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资助金额:$2.18万
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财政年份:2011
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依托单位:
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财政年份:2011
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负责人:STANLEY FIELDS
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财政年份:2010
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负责人:STANLEY FIELDS
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依托单位:
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