DESIGN OF SELF-ASSEMBLED 3D DNA CRYSTALS USING 6HB

使用 6HB 设计自组装 3D DNA 晶体

• 批准号: 7957272
• 负责人: JENS J BIRKTOFT
• 金额: $ 1.88万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957272
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; Devices; Diffusion; Electronics; Funding; Goals; Grant; Institution; Light; Methods; Molecular; Nanotechnology; Pattern; Positioning Attribute; Proteins; Research; Research Personnel; Resolution; Resources; Shapes; Source; Synchrotrons; System; United States National Institutes of Health; Work; X ray diffraction analysis; X-Ray Diffraction; base; design; macromolecule; nanoscale; success; three dimensional structure; vapor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural DNA nanotechnology uses unusual DNA motifs to build target shapes and periodic arrangements. We expect these systems can be applied to several practical ends: The key motivating goal for this research is that spatially periodic networks are crystals. Also the Steepest challenge of the research is the construction of 3D crystals with high order. If we can build stick-figure crystalline cages in the nanometer scale, they could be used to orient other biological macromolecules as guests inside those cages, thereby rendering their 3D structure amenable to diffraction analysis. The globular shapes of proteins are not conducive to the packing alignment that is essential to form a well-ordered crystal, due to specific interactions between DNA and proteins, DNA 3D crystalline crystal is an excellent candidate for forming periodic crystalline cages that can host proteins in order for the X-ray diffraction studies; Similarly, the same crystalline arrays could be used to position and orient components of molecular electronic devices with nanometer-scale precision. A number of designs using unusual DNA motifs have been self-assembled to yield 3D crystals. Although we have been successfully build 2D arrays, the structural criteria of success with 2D arrangements are based typically on AFM observation with resolution limit of 3-10 nm. By contrast the goal for successful work of x-ray diffraction is around 2 ¿¿¿ . However the diffraction patterns of our preliminary crystals have been limited to 10 ¿¿¿ . Six-DNA helix-Bundle is the exact motif I¿¿¿¿¿"m using for this research. In this motif six DNA helices associate to form a hexagon arrangement if you look down the helical axis of DNA. We use thermo-control and vapour-diffusion methods. We have been successfully made 2D arrays by using the former method.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 结构DNA纳米技术使用不寻常的DNA基序来构建目标形状和周期性排列。我们希望这些系统可以应用于几个实际目的：这项研究的关键动机目标是空间周期性网络是晶体。此外，该研究最大的挑战是构建高阶3D晶体。如果我们能在纳米尺度上构建棒状晶体笼，它们就可以用来定向其他生物大分子作为这些笼内的客人，从而使它们的3D结构适合衍射分析。由于DNA和蛋白质之间的特异性相互作用，蛋白质的球形形状不利于形成有序晶体所必需的堆积排列，DNA 3D晶体是形成周期性晶体笼的极好候选者，所述周期性晶体笼可以容纳蛋白质以用于X射线衍射研究;类似地，同样的晶体阵列可以用于以纳米级精度定位和定向分子电子器件的组件。 许多使用不寻常的DNA基序的设计已经被自组装成3D晶体。虽然我们已经成功地构建了2D阵列，但2D排列成功的结构标准通常基于分辨率限制为3-10 nm的AFM观察。相比之下，成功的X射线衍射工作的目标是大约2。然而，我们的初步晶体的衍射图案已被限制在10。六DNA螺旋束正是我在这项研究中使用的基序。在这个基序中，如果你沿着DNA的螺旋轴向下看，六个DNA螺旋结合形成一个六边形排列。我们使用温度控制和蒸汽扩散方法。我们已经成功地利用前一种方法制作了二维阵列。