PROTEINS INVOLVED WITH NITRIC OXIDE SIGNALING, RIBOFLAVIN SYNTHESIS AND COPPER H

与一氧化氮信号传导、核黄素合成和铜 H 相关的蛋白质

• 批准号: 7954309
• 负责人: SUE L ROBERTS
• 金额: $ 0.21万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954309
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Copper; Family; Freezing; Funding; GTP Cyclohydrolase II; Glutathione; Grant; Heme; Heme Iron; Hemeproteins; Institution; Modeling; Molecular Conformation; Myoglobin; Nitric Oxide; Nitrosation; Oxidases; Oxidoreductase; Proteins; Reaction; Research; Research Personnel; Resolution; Resources; Riboflavin; SKIL gene; Signal Transduction; Signaling Protein; Source; Structure; Thioredoxin; Time; United States National Institutes of Health; adduct; base; drug discovery; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are requesting beam time to further four projects. First we are continuing our studies on nitrosylated heme proteins, including the nitrophorins and other heme-based NO signaling proteins. Second, we have undertaken a wide-ranging study of protein S-nitrosation in NO signaling, including S-NO formation (thioredoxin, myoglobin), and control of glutathione S-nitrosation by the protein GSNO reductase. These studies include a drug discovery component. Third, we are investigating proteins involved in riboflavin synthesis in S. coleicolor, particularly the GTP cyclohydrolase II family. Fourth, we are pursuing reaction intermediates in several proteins, including the multicopper oxidase CueO. Synchrotron radiation is needed for: (1) MAD structure determination; (2) Sufficient resolution to determine accurate geometry of SNO adducts, define heme distortion from planarity, model multiple conformations; and determine the structure of freeze-trapped intermediates in active crystals; (3) Variable x-ray wavelengths can be used to minimize photoreduction of iron-heme centers.

这个子项目是众多研究子项目之一