PROTEIN CRYSTAL STRUCTURE DETERMINATIONS RELATED TO NITRIC OXIDE SIGNALING AND O

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们要求光束时间，以进一步几个项目。首先，我们正在研究亚硝化的血红素蛋白，包括亚硝酚和基于亚铁血红素的NO信号蛋白，特别是可溶性鸟苷环化酶(SGC)。第二，我们正在继续研究蛋白质S亚硝化，以及通过蛋白质GSnO还原酶控制谷胱甘肽S亚硝化。GSNOR研究包括药物发现部分。第三，我们正在研究与核黄素合成有关的蛋白质，特别是GTP环水解酶。第四，我们正在扩大对参与细菌金属运输和动态平衡的蛋白质的研究，重点是CusF、SILF、CusB和CueO。最后，我们将与Matt Cordes合作，使用传递性同源性研究指导的结构确定来绘制Cro蛋白和Lipocalin的折叠进化图。水晶可用于所有项目。有些衍射率为1.0或更高。同步辐射用于(1)MAD结构测定，(2)提供足够的分辨率来确定准确的几何参数，如从平面性和金属结合位几何形状扭曲的血红素；(3)提供可变波长的X射线，用于明确识别结合的金属原子，并控制金属位和S-NO键的光还原。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We request beam time to further several projects. First, we are studying nitrosylated heme proteins, including nitrophorins and heme-based NO signaling proteins, particularly soluble guanylate cyclase (sGC). Second, we are continuing studies of protein S-nitrosation, and control of glutathione S-nitrosation by the protein GSNO reductase. The GSNOR studies include a drug discovery component. Third, we are investigating proteins involved in riboflavin synthesis, particularly the GTP cyclohydrolases. Fourth, we are expanding studies of proteins involved in metal transport and homeostasis in bacteria, focusing on cusF, silF, cusB, and cueO. Finally, we will, in collaboration with Matt Cordes, use structure determinations guided by transitive homology studies to map fold evolution of Cro proteins and lipocalins. Crystals are available for all projects. Some diffract to 1.0 ¿ or better. Synchrotron radiation is needed for (1) MAD structure determination, (2) to provide sufficient resolution to determine accurate geometrical parameters such as heme distortion from planarity and metal binding site geometries; (3) to provide variable wavelength x-rays for unambiguous identification of bound metal atoms and to control photoreduction of metal sites and S-NO bonds.