STRUCTURE OF A BACTERIA TIR CONTAINING VIRULENCE FACTOR

含有毒力因子的细菌 TIR 的结构

• 批准号: 7954472
• 负责人: LIEH YOON LOW
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954472
• 关键词: Bacteria; Computer Retrieval of Information on Scientific Projects Database; Data; Family; Funding; Grant; Institution; Length; Methods; Molecular Conformation; N-terminal; Research; Research Personnel; Resources; Signal Transduction; Source; Structure; United States National Institutes of Health; Virulence Factors; dimer; factor A; insight; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to get insights into the function of this new family of virulence factors it is crucial to ascertain the conformation of the TIR domain inside the signaling active full length homodimer. We expect SAXS data to give us the orientation of the dimer: is the TIR domain opposing the TIR of the partner molecule or the N-terminal helical region? Having solved the structure of the TIR domain, our top priority is to find a quick method that could provide empirical evidence of the dimer organization because of its functional relevance.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为了深入了解这一新毒力因子家族的功能，确定信号活性全长同二聚体内 TIR 结构域的构象至关重要。我们期望 SAXS 数据能够为我们提供二聚体的方向：TIR 结构域是否与伙伴分子的 TIR 或 N 末端螺旋区域相对？解决了 TIR 结构域的结构后，我们的首要任务是找到一种快速方法，由于其功能相关性，可以提供二聚体组织的经验证据。