CRYSTALLOGRAPHIC STUDIES OF VIRULENCE FACTORS, HOST-PATHOGEN INTERACTIONS, AND O

毒力因子、宿主-病原体相互作用和 O 的晶体学研究

• 批准号: 7954520
• 负责人: LIEH YOON LOW
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954520
• 关键词: Collection; Collimator; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Dedications; Disease; Drug Design; Funding; Future; Grant; Immune; Inflammation; Institution; Integration Host Factors; Laboratories; Phase; Play; Proteins; Reporting; Research; Research Personnel; Resolution; Resources; Role; Science; Source; Structure; Support Groups; Therapeutic; United States National Institutes of Health; Virulence Factors; Work; anthrax toxin; cell motility; design; inhibitor/antagonist; neutralizing antibody; pathogen; programs; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a continuing proposal for the Liddington laboratory, describing our overall program in structural biology at the SSRL. The major focuses are anthrax toxin and virulence factors from other high priority pathogens and emerging diseases, and their interactions with host factors, as well as host proteins involved in cell migration and inflammation. The work involves ab initio structure determination by MAD phasing, studies of protein-inhibitor complexes for rational drug design, and complexes with neutralizing antibodies for designing immune therapeutics. I report highlights of our studies for the 2005-2008 period in the context of our earlier work at SSRL, as well as our ongoing and future studies. This work illustrates how the SSRL has played a central and critical role in most aspects of our science ? using its tunability for primary structure determination/phasing; and/or the high brightness, collimation and stability of the SSRL source for the collection of high resolution data from demanding crystals. And all of this made possible and much more pleasant through the dedication of the outstanding User Support Group.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这是 Liddington 实验室的一项持续提案，描述了我们 SSRL 在结构生物学方面的总体计划。主要关注点是来自其他高优先级病原体和新出现疾病的炭疽毒素和毒力因子，以及它们与宿主因子以及参与细胞迁移和炎症的宿主蛋白的相互作用。这项工作涉及通过 MAD 定相从头开始确定结构、研究用于合理药物设计的蛋白质抑制剂复合物以及用于设计免疫疗法的中和抗体复合物。我在 SSRL 早期工作以及我们正在进行和未来的研究的背景下报告了我们 2005-2008 年期间的研究要点。这项工作说明了 SSRL 如何在我们科学的大多数方面发挥核心和关键作用？利用其可调性进行一级结构确定/定相；和/或 SSRL 源的高亮度、准直和稳定性，用于从要求苛刻的晶体中收集高分辨率数据。通过杰出的用户支持小组的奉献，所有这一切都成为可能并且更加令人愉快。