STRATEGY CALCULATION FOR PHASING PROTEIN CRYSTAL STRUCTURES IN THE PRESENCE OF R

R 存在下定相蛋白质晶体结构的策略计算

• 批准号: 7954182
• 负责人: ANA M GONZALEZ
• 金额: $ 0.29万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954182
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Data Collection; Data Quality; Disulfides; Elements; Funding; Grant; Institution; Muramidase; Phase; Proteins; Radiation; Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; research study; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although there is evidence that the energy chosen for data collection does not have a great deal of effect in the global quality of the data [Shimizu, Hirata, Hasegawa, Ueno & Yamamoto (2007) J. Synchrotron Radiat. 14 4-10], the effects of local radiation damage at different energies have not been sufficiently studied. Experiments have been undertaken to study the reduction rate of disulfide bridges at 9 and 14.5 keV on lysozyme. The damage to disulfide bridges happens relatively quickly and it is easy to observe and quantify, and the radiation damage mechanism is thought to be the same as for other elements. Preliminary results indicate that the rate of reduction of the disulfide bridges is site depending, with more exposed sites showing an increased rate of decay and there is no apparent difference in the rate of decay at the two energies chosen for the study.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 尽管有证据表明，选择用于数据收集的能量对数据的整体质量没有很大影响[Shimizu，Hirata，Hasegawa，Ueno & Yamamoto（2007）J. Synchrotron Radiat. 14 4-10]，不同能量下局部辐射损伤的影响尚未得到充分研究。 实验研究了溶菌酶在9和14.5keV下二硫键的还原速率。 二硫键的损伤发生相对较快，易于观察和量化，辐射损伤机制被认为与其他元素相同。初步结果表明，二硫键的还原率是站点依赖性的，更多的暴露站点显示出增加的衰变率，并且在选择用于研究的两种能量下的衰变率没有明显差异。