PERSONALIZED MACROMOLECULAR CRYSTALLOGRAPHY USER WORKSHOPS

个性化高分子晶体学用户研讨会

• 批准号: 8362102
• 负责人: ANA M GONZALEZ
• 金额: $ 1.37万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362102
• 关键词: Crystallography; Data; Data Collection; Educational workshop; Equipment; Fluorescence; Funding; Grant; Ice; Internet; National Center for Research Resources; Participant; Principal Investigator; Process; Radiation; Research; Research Infrastructure; Resources; Sampling; Scanning; Screening procedure; Shipping; Ships; Source; System; Training; United States National Institutes of Health; cost; research study; structural biology; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Macromolecular crystallography user training workshops are held at SSRL to familiarize users who would like to collect data remotely with the available tools and processes or to provide in-depth individualized training on the beam lines. Three workshops were held this year. Attendees are introduced to the experimental equipment and the computing facilities available both locally and remotely. Participants are instructed on how to effectively use Blu-Ice, Web-Ice and the remote access tools. Training topics include 1) how to prepare and ship samples for use with the Stanford Automated Mounting (SAM) system, 2) automated crystal screening and data collection, 3) fluorescence scans and MAD experiments, and 4) strategy determination for data collection using Web-Ice.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在SSRL举办了大分子结晶学用户培训讲习班，以使希望远程收集数据的用户熟悉现有的工具和流程，或在光束线上提供深入的个性化培训。今年举办了三个讲习班。向与会者介绍了本地和远程可用的实验设备和计算设施。指导学员如何有效使用Blu-Ice、Web-Ice和远程访问工具。培训主题包括1)如何准备和运输样品以用于斯坦福自动安装(SAM)系统，2)自动晶体筛选和数据收集，3)荧光扫描和MAD实验，以及4)使用Web-Ice收集数据的策略确定。