FEASIBILITY TESTS FOR WIDE ANGLE FIBER DIFFRACTION STUDIES ON 14 BM-C

14 BM-C 广角光纤衍射研究的可行性测试

• 批准号: 7956803
• 负责人: Joseph Patrick Rosen O'Dubhthaigh Orgel
• 金额: $ 1.41万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956803
• 关键词: Amyloidosis; Blood capillaries; Collagen; Collagen Type I; Collagen Type II; Computer Retrieval of Information on Scientific Projects Database; Connective Tissue; Fiber; Freezing; Funding; Grant; In Situ; Institution; Lampreys; Mica; Pattern; Peptides; Perspex; Prion Diseases; Radiation; Rattus; Research; Research Personnel; Resources; Sampling; Source; System; Tail; Temperature; Tendon structure; Testing; United States National Institutes of Health; beamline; biological systems; capillary; notochord; research study; seal; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to continue to evaluate 14 BM-C for the purpose of collecting x-ray patterns from fibrous biological systems. At present, 18 ID is the only beamline at the APS that routinely offers wide-angle fiber diffraction. While many of these kinds of samples benefit from the high beam quality of doubly focussed undulator radiation, these experiments are not flux limited and a large fraction of them may well be adequately done on an appropriately configured bending magnet line. High quality wide angle fiber patterns can be taken of 14 BM-C, however, we need to continue to characterize the range of experiments that are possible at this facility (d-space range and quality of diffraction from representative test systems). If successful, 14BM-C would provide a significant increase in capacity for this kind of experiment at the APS. Furthermore, a class of biomedically important problems that can be studied by fiber diffraction is amyloidosis and prion diseases. Many of these samples are biohazardous and need to be studied under Biosafety level 2 and/or 3 conditions. We will also evaluate the setup for connective tissue studies. Type II collagen in situ in lamprey notochord will be mounted in sealed (perspex/mica) fibre sample holders. Type I collagen in situ in rat-tail tendon will be mounted in sealed (perspex/mica) fiber sample holders. Collagen short peptide crystals will be mounted in sealed capillaries. Collagenous samples may also be cryo-frozen in the same manner that single crystals are. Patterns will be compared to those taken on 18ID. All samples will studied at room temperature and are non-hazardous.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是继续评价14种BM-C，以收集纤维生物系统的X射线模式。目前，18 ID是APS唯一一条常规提供广角光纤衍射的光束线。虽然这些种类的样品中的许多受益于双聚焦波荡器辐射的高光束质量，但这些实验不受通量限制，并且它们中的大部分可以在适当配置的弯曲磁体线上充分地完成。可以从14 BM-C获得高质量的广角光纤图案，但是，我们需要继续表征该设施可能的实验范围（代表性测试系统的d空间范围和衍射质量）。 如果成功，14BM-C将为APS的此类实验提供显着的能力增加。此外，可以通过纤维衍射研究的一类重要生物医学问题是淀粉样变性和朊病毒疾病。这些样品中有许多是生物危害性的，需要在生物安全2级和/或3级条件下进行研究。我们还将评估结缔组织研究的设置。II型胶原蛋白在七鳃鳗脊索原位将安装在密封（有机玻璃/云母）纤维样品架。将大鼠尾肌腱中的原位I型胶原固定在密封（有机玻璃/云母）纤维样品架中。胶原短肽晶体将固定在密封的毛细管中。胶原样品也可以以与单晶相同的方式冷冻。将模式与18ID上的模式进行比较。所有样品均在室温下进行研究，且无危害。