MICRO WIDE ANGLE FIBER DIFFRACTION DEVELOPMENT AND CHARACTERIZATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本赠款周期的核心项目之一是开发一种灵活的高通量仪器，用于生物材料中小有序域的微衍射，重点是结缔组织和神经组织的研究。微衍射能力应满足以下要求：对于广角：d-空间范围200-3.0？，以解析淀粉样蛋白交叉β特征和来自超螺旋的任何特征的高次谐波。对于小角度，d空间范围为800-100，以解析胶原蛋白的第一级（670），与WAX数据重叠，以合并低角度和高角度数据，并解析Tau纤维螺旋扭曲（~800）。样品上的焦斑尺寸在每个方向上需要小于10微米（优选地小得多）。使用低温结晶设备对于微衍射模式将是极其重要的，以防止样品在长时间暴露于X射线束时受到辐射损伤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the core projects in the current grant cycle is the development of a flexible, high flux instrument for micro-diffraction of small ordered domains in biological materials with emphasis on studies of connective and neurological tissue. The micro-diffraction capabilities should meet the following requirements: For wide angle: d-space range 200-3.0 ¿ to resolve amyloid cross-beta signatures and higher harmonics of any features from super-helices. For small angle a d-space range of 800-100 ¿ to resolve first order of collagen (670) with overlap with WAX data for merging of low and high angle data and resolution of Tau fiber helical twist (~800 ¿). The focal spot sizes, on the sample, need to be less than 10 microns (preferably much less) in each direction. Use of cryo-crystallography equipment will be extremely important for the micro-diffraction modes, to prevent radiation damage in the sample while exposed to the X-ray beam over extended periods of time.