FEASIBILITY TESTS FOR WIDE ANGLE FIBER DIFFRACTION STUDIES ON 14 BM-C

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究的目的是继续评估14 BM-C，目的是从纤维生物系统收集X射线模式。目前，18ID是APS唯一一条常规提供广角光纤衍射的光束线。虽然许多这类样品受益于双聚焦波荡器辐射的高光束质量，但这些实验不受通量限制，其中很大一部分很可能在适当配置的弯曲磁力线上完成。14BM-C可以获得高质量的广角光纤图案，然而，我们需要继续表征在该设施中可能进行的实验范围(d-空间范围和来自典型测试系统的衍射质量)。如果成功，14BM-C将为APS的这类实验提供显著增加的能力。此外，一类重要的生物医学问题可以通过纤维衍射来研究，这类问题是淀粉样变性和普里恩病。其中许多样本具有生物危害性，需要在生物安全级别2和/或3的条件下进行研究。我们还将评估结缔组织研究的设置。鳗鱼脊索中原位的II型胶原将安装在密封的(有机玻璃/云母)纤维样品盒中。鼠尾肌腱中原位的I型胶原将安装在密封的(有机玻璃/云母)纤维样品支架中。胶原蛋白短肽晶体将被安装在密封的毛细血管中。胶原蛋白样品也可以用与单晶相同的方式冷冻。图案将与18ID拍摄的图案进行比较。所有样品都将在室温下进行研究，并且是无害的。我们预计完成植物病毒研究需要三个班次，结缔组织研究需要额外三个班次。我们估计，除了为这些研究制定最佳实验配置外，可能还需要三个班次。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to continue to evaluate 14 BM-C for the purpose of collecting x-ray patterns from fibrous biological systems. At present, 18 ID is the only beamline at the APS that routinely offers wide-angle fiber diffraction. While many of these kinds of samples benefit from the high beam quality of doubly focussed undulator radiation, these experiments are not flux limited and a large fraction of them may well be adequately done on an appropriately configured bending magnet line. High quality wide angle fiber patterns can be taken of 14 BM-C, however, we need to continue to characterize the range of experiments that are possible at this facility (d-space range and quality of diffraction from representative test systems). If successful, 14BM-C would provide a significant increase in capacity for this kind of experiment at the APS. Furthermore, a class of biomedically important problems that can be studied by fiber diffraction is amyloidosis and prion diseases. Many of these samples are biohazardous and need to be studied under Biosafety level 2 and/or 3 conditions. We will also evaluate the setup for connective tissue studies. Type II collagen in situ in lamprey notochord will be mounted in sealed (perspex/mica) fibre sample holders. Type I collagen in situ in rat-tail tendon will be mounted in sealed (perspex/mica) fiber sample holders. Collagen short peptide crystals will be mounted in sealed capillaries. Collagenous samples may also be cryo-frozen in the same manner that single crystals are. Patterns will be compared to those taken on 18ID. All samples will studied at room temperature and are non-hazardous. We anticipate that it would take three shifts to complete the plant virus studies and three additional shifts for the connective tissue studies. We estimate that it may take three shifts in addition to work out the best experimental configuration for these studies.