RECOGNITION EVENTS IN RNA INTERFERENCE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肌肉盲样蛋白(MBNL)是一种发育程序性选择性剪接的调节蛋白，具有串联CCCH锌指(ZnF)结构域，靶向含有YGCU(U/G)Y序列元件的前-mRNAs。在强直性肌营养不良症中，MBNL蛋白水平的降低导致前mRNAs子集的异常选择性剪接。为了阐明MBNL识别调控RNA元件的原理，我们将每个MBNL1串联的锌F结构域，即锌F1/2和锌氟3/4，以自由形式结晶，并将第二个串联的锌氟3/4与CGCUGU核苷酸结合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Muscleblind-like (MBNL) proteins, regulators of developmentally programmed alternative splicing, harbor tandem CCCH Zn finger (ZnF) domains, that target pre-mRNAs containing YGCU(U/G)Y sequence elements. In myotonic dystrophy, reduced levels of MBNL proteins lead to aberrant alternative splicing of a subset of pre-mRNAs. To elucidate principles of the regulatory RNA elements recognition by MBNL we crystallized each MBNL1 tandem ZnF domain, ZnF1/2 and ZnF3/4, in the free form, and the structure of the second tandem ZnF3/4 bound to CGCUGU nucleotide.