RECOGNITION EVENTS IN RNA INTERFERENCE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 肌盲样（MBNL）蛋白是发育程序性选择性剪接的调节剂，具有串联CCCH锌指（ZnF）结构域，其靶向含有YGCU（U/G）Y序列元件的前mRNA。在强直性肌营养不良中，MBNL蛋白水平降低导致前体mRNA亚组的异常选择性剪接。为了阐明MBNL识别调控RNA元件的原理，我们以游离形式结晶每个MBNL 1串联ZnF结构域，ZnF 1/2和ZnF 3/4，以及与CGCUGU核苷酸结合的第二串联ZnF 3/4的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Muscleblind-like (MBNL) proteins, regulators of developmentally programmed alternative splicing, harbor tandem CCCH Zn finger (ZnF) domains, that target pre-mRNAs containing YGCU(U/G)Y sequence elements. In myotonic dystrophy, reduced levels of MBNL proteins lead to aberrant alternative splicing of a subset of pre-mRNAs. To elucidate principles of the regulatory RNA elements recognition by MBNL we crystallized each MBNL1 tandem ZnF domain, ZnF1/2 and ZnF3/4, in the free form, and the structure of the second tandem ZnF3/4 bound to CGCUGU nucleotide.