NORTHEAST STRUCTURAL GENOMICS CONSORTIUM

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 位于马里兰州贝塞斯达的美国国立卫生研究院（NIH） 蛋白质结构倡议（PSI-2）其最终目标是 来获得一万个蛋白质的三维结构。就像人类基因组 这项工作可以改变我们对基本生物学的理解， 流程. NIH指定了4个中心来制造蛋白质结构。之一 东北结构基因组学联盟（Northeast Structural Genomics Consortium，NESGC）由9个 不同的组织。NESGC中有两种方法提供3D 蛋白质结构：X射线晶体学和核磁共振 （NMR）。哥伦比亚大学是X射线蛋白的基本贡献者 结构.通过X射线获得结构的快速方法是使用SeMet 野生型蛋白的衍生物。 这是在 哥伦比亚大学生物科学系 根据这些数据成功地确定了ET 72的晶体结构，并已存入蛋白质数据库（代码1 YW 6）。 ET 72是来自E.杆菌

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The U.S. National Institute of Health (NIH) in Bethesda, Maryland, launched the second phase of Protein Structure Initiative (PSI-2). Its ultimate goal is to obtain 3-dimensional structures of 10,000 proteins. Like the Human Genome Project, this effort could transform our understanding of basic biological processes. NIH designated 4 centers to crank out protein structures. One of them is Northeast Structural Genomics Consortium (NESGC) consists of 9 different organizations. There are two approaches in the NESGC to provide 3D protein structures: X-Ray crystallography and Nuclear Magnetic Resonance (NMR). Columbia University is the basic contributor of the X-ray protein structures. The fast way to obtain the structure by X-ray is to use SeMet derivatives of the wild-type proteins. This is the main method used in the Columbia University, Department of Biological Sciences The crystal structure of ET72 has been successfully determined based on the data, and has been deposited in the Protein Data Bank (code 1YW6). ET72 is a succinylglutamate desuccinylase from E. coli.