PROTEOMICS OF LIFE SPAN EXTENSION IN C ELEGANS

线虫寿命延长的蛋白质组学

• 批准号: 7957020
• 负责人: RENE A BRAECKMAN
• 金额: $ 3.25万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957020
• 关键词: Affect; Age; Aging; Aging-Related Process; Biology; Caenorhabditis elegans; Computer Retrieval of Information on Scientific Projects Database; Data; Degradation Pathway; Down-Regulation; Funding; Future; Genetic; Goals; Grant; Institution; Knowledge; Laboratories; Life; Longevity; Mitochondria; Modeling; Molecular; Morphology; Mutation; National Center for Research Resources; Nematoda; Organism; Physiological; Physiology; Pilot Projects; Process; Proteins; Proteome; Proteomics; Research; Research Personnel; Resources; Route; Signal Pathway; Source; United States National Institutes of Health; dietary restriction; imprint; mutant; protein degradation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The C. elegans nematode worm is a very short-living multicellular organism with a lifespan of only 2-3 weeks in optimal laboratory conditions. Such a short lifespan, in addition to accumulated wealth of knowledge about the physiology, morphology and genetics of this organism makes it one of the most convenient models to study the fundamental mechanisms of aging. It has been shown that the lifespan of wild-type C. elegans can be extended drastically in a number of seemingly independent ways. For example it can be extended up to several fold by dietary restriction, by mutation of the Ins/IGF signaling pathway, or by down-regulation of mitochondrial function. Nonetheless, despite the accumulated physiological and primarily genetic data, the molecular mechanisms behind the aging phenomenon and lifespan extension have not been fully outlined yet. In this NCRR collaborative proposal we want to move beyond approaches that have been traditionally used for studying C. elegans aging and take a detailed look at the proteome. Here, in a pilot study, we propose to look at the proteome changes over the age for normally lived worms, long-lived mutant worms and dietary restricted worms. Our goal is to find common downstream proteins or common imprints of certain processes (like specific protein degradation pathways) affecting longevity. This new alternative look at the C. elegans aging process from a proteomics perspective may yield new routes for future research that are not biased by existing aging hypotheses.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 线虫是一种寿命非常短的多细胞生物，在最佳实验室条件下其寿命仅为 2-3 周。 如此短暂的寿命，加上积累了丰富的有关该生物体的生理学、形态学和遗传学知识，使其成为研究衰老基本机制的最方便的模型之一。 研究表明，野生型线虫的寿命可以通过多种看似独立的方式大幅延长。 例如，通过饮食限制、Ins/IGF信号通路突变或线粒体功能下调，它可以延长数倍。 尽管如此，尽管积累了生理和主要遗传数据，但衰老现象和寿命延长背后的分子机制尚未完全阐明。 在这项 NCRR 合作提案中，我们希望超越传统上用于研究线虫衰老的方法，并详细研究蛋白质组。 在这里，在一项试点研究中，我们建议观察正常生活的蠕虫、长寿突变蠕虫和饮食限制蠕虫的蛋白质组随着年龄的变化。 我们的目标是找到影响寿命的常见下游蛋白质或某些过程（如特定蛋白质降解途径）的常见印记。 这种从蛋白质组学角度观察线虫衰老过程的新方法可能会为未来的研究带来新的途径，而不受现有衰老假设的影响。