Mouse Phenotype Core, Project 9 of 10

肥胖的不良后果对哺乳动物生物体是多效性的。动物模型 肥胖症，允许仔细表征疾病的普遍代谢影响是至关重要的， 我们使命的成功为了加强对肥胖原因和后果的研究，我们建立了 小鼠表型分析核心。这个核心的主要目标是开发和提供标准化的状态， 用于分析疾病的动物模型的现有技术方法。核心应用了10个 实验室提供组织学，神经解剖学，基因表达，质谱，生理学，脂质， 葡萄糖、代谢因子和行为测定。核心是由罗素，埃尔姆奎斯特，和谢勒， 配备专业的熟练技术人员团队，他们利用现有设备进行优化分析 TORS调查人员提供的样本。目前可用的测定包括组织学染色和免疫组织化学。 抗原检测、原位mRNA杂交、真实的时间反转录酶-PCR、微阵列分析， 激光捕获显微切割，小分子质谱和代谢物分析，代谢笼 分析，完整的脂质平衡研究，磁共振和计算机断层扫描成像，葡萄糖 耐受性和钳夹分析、血清激素测量、常规血液化学、详尽的 行为分析和脑切片中的突触可塑性测量。新的研究发现表明， 核心小组开发并提供新的方法。对于每个实验，结果是 以电子格式收集、计算和编辑，并返回给提交研究者。的 该核心提供的服务的可用性加快了我们研究的步伐，允许集中 故障排除，并促进有效利用现有的专业知识和资源。鼠标 表型核心是我们路线图工作的重要组成部分，它汇集了一个多元化的团队， 科学家和临床医生，以确保肥胖研究中的发现迅速转化为 病人护理的进步。

The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.