Mouse Phenotype Core, Project 9 of 10

小鼠表型核心，项目 9（共 10 个）

• 批准号: 8009166
• 负责人: DAVID W. RUSSELL
• 金额: $ 9.99万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8009166
• 关键词: Affect; Amino Acid Sequence; Animal Disease Models; Animal Model; Antibody Formation; Antigens; Arts; Behavioral; Behavioral Assay; Biological Assay; Blood Chemical Analysis; Brain; Collaborations; Consult; Core Facility; Crystallography; DNA Sequence; Data; Detection; Disease; Electronics; Ensure; Equilibrium; Equipment; Faculty; Fee-for-Service Plans; Flow Cytometry; Funding; Gene Expression; Gene Targeting; Glucose; Goals; Grant; Guidelines; Health Services Accessibility; Histology; Home environment; Hormones; Image; In Situ; Kinetics; Knowledge; Laboratories; Life; Lipids; Magnetic Resonance; Mass Spectrum Analysis; Measurement; Messenger RNA; Metabolic; Methodology; Methods; Microarray Analysis; Mission; Mus; Neuroanatomy; Obesity; Organism; Patient Care; Peptide Sequence Determination; Phenotype; Physiological; Physiology; Procedures; Production; Quality Control; RNA chemical synthesis; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Running; Sampling; Scientist; Serum; Services; Slice; Staging; Staining method; Stains; Study of magnetics; Synaptic plasticity; Testing; Time; Transgenic Animals; Translating; Work; X-Ray Computed Tomography; adverse outcome; analytical ultracentrifugation; base; body system; cellular imaging; glucose tolerance; insight; interest; laser capture microdissection; member; novel; research study; small molecule; structural biology; success; web site

The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.

肥胖的不利后果是哺乳动物生物的多效性。动物模型 允许对疾病普遍代谢作用进行仔细表征的肥胖症对 我们的使命的成功。为了加强对肥胖的原因和后果的研究，我们建立了 鼠标表型核心。该核心的主要目标是开发和提供标准化的最先进 用于分析疾病模型的艺术方法。核心应用十个分析专业知识 提供组织学，神经解剖学，基因表达，质谱，生理学，脂质，脂质， 葡萄糖，元代表和行为测定。核心由罗素，埃尔姆奎斯特和谢勒指导，是 由专门的熟练技术人员团队组成，他们利用现有设备进行优化测定 关于TORS调查人员提供的样品。目前可用的测定包括组织学染色和 抗原检测，原位mRNA杂交，实时逆转录酶-PCR，微阵列分析， 激光捕获显微解剖，小分子质谱法和代谢物分析，代谢笼 分析，完整的脂质平衡研究，磁共振和计算机断层扫描成像，葡萄糖 耐受性和夹具分析，血清激素测量，常规血液化学，详尽 行为分析和脑切片中的突触可塑性测量。正如新的研究发现所指出的那样， 新方法是由核心开发并提供的。对于每个实验，结果是 以电子格式收集，计算和编译，并返回提交的调查人员。这 该核心提供的服务的可用性加速了我们的研究步伐，允许集中 故障排除，并促进现有专业知识和资源的有效利用。鼠标 表型核心是我们路线图工作的关键组成部分，它汇集了一个多样化的团队 科学家和临床医生确保肥胖研究中的发现迅速转化为 患者护理的进步。