Mouse Phenotype Core, Project 9 of 10

小鼠表型核心，项目 9（共 10 个）

• 批准号: 8009166
• 负责人: DAVID W. RUSSELL
• 金额: $ 9.99万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8009166
• 关键词: Affect; Amino Acid Sequence; Animal Disease Models; Animal Model; Antibody Formation; Antigens; Arts; Behavioral; Behavioral Assay; Biological Assay; Blood Chemical Analysis; Brain; Collaborations; Consult; Core Facility; Crystallography; DNA Sequence; Data; Detection; Disease; Electronics; Ensure; Equilibrium; Equipment; Faculty; Fee-for-Service Plans; Flow Cytometry; Funding; Gene Expression; Gene Targeting; Glucose; Goals; Grant; Guidelines; Health Services Accessibility; Histology; Home environment; Hormones; Image; In Situ; Kinetics; Knowledge; Laboratories; Life; Lipids; Magnetic Resonance; Mass Spectrum Analysis; Measurement; Messenger RNA; Metabolic; Methodology; Methods; Microarray Analysis; Mission; Mus; Neuroanatomy; Obesity; Organism; Patient Care; Peptide Sequence Determination; Phenotype; Physiological; Physiology; Procedures; Production; Quality Control; RNA chemical synthesis; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Running; Sampling; Scientist; Serum; Services; Slice; Staging; Staining method; Stains; Study of magnetics; Synaptic plasticity; Testing; Time; Transgenic Animals; Translating; Work; X-Ray Computed Tomography; adverse outcome; analytical ultracentrifugation; base; body system; cellular imaging; glucose tolerance; insight; interest; laser capture microdissection; member; novel; research study; small molecule; structural biology; success; web site

The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.

肥胖对哺乳动物机体的不良后果是多方面的。动物模型