Mouse Phenotype Core

肥胖的不良后果对哺乳动物有机体来说是多种多样的。大鼠的动物模型 能够仔细描述这种疾病普遍存在的新陈代谢影响的肥胖对 我们的任务成功了。为了加强对肥胖原因和后果的研究，我们建立了 一个小鼠的表型核心。这个核心的主要目标是开发和提供标准化的状态-- 疾病动物模型分析的ART方法。核心应用了十个方面的分析专业知识 提供组织学、神经解剖学、基因表达、质谱学、生理学、脂类、 葡萄糖、代谢物和行为分析。核心由Russell，Elmquist和Scherer执导，是 配备了一支专门的技术人员团队，他们利用现有的设备进行优化的分析 由Tors调查人员提供的样本。目前可用的检测方法包括组织学染色和 抗原检测、原位mRNA杂交、实时逆转录聚合酶链式反应、基因芯片分析、 激光捕获显微解剖，小分子质谱学和代谢物图谱，代谢笼 分析，完整的脂平衡研究，磁共振和计算机断层成像，葡萄糖 耐受性和钳制分析，血清激素测量，常规血液化学，穷尽 行为分析和脑片突触可塑性测量。正如新的研究结果所表明的， 核心开发了新的方法并使之可用。对于每个实验，结果是 以电子格式收集、计算和汇编，并返回给提交调查人员。这个 该核心提供的服务的可用性加快了我们的研究步伐，允许集中 故障排除，并促进有效利用现有的专业知识和资源。鼠标 表型分析核心是我们路线图工作的重要组成部分，它将不同的团队聚集在一起 科学家和临床医生确保肥胖研究的发现迅速转化为 病人护理方面的进展。

The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.