FLOW CYTOMETRY ANALYSIS/ HIGH SPEED CELL SORTING

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 荧光细胞计数核心设施的功能是为研究人员提供最先进的技术，以帮助他们了解环境健康挑战所涉及的细胞过程。 了解这一层次的过程使研究人员能够制定和测试理论，以环境因素如何影响健康。 这些发现对于疾病预防和治疗的探索非常重要。 Core是使用荧光标记物和/或单克隆抗体来提供照亮细胞过程的数据的仪器的所在地。 BD FACSAria "是最先进的流式细胞仪和高速细胞分选仪。 三个激光器，九个滤波器组配置允许同时分析多个分子信号的识别。 此外，该仪器具有对感兴趣的亚群进行精确分选以用于进一步研究的能力。BD FACSCalibur "是一款单激光、三检测器流式细胞仪，可提供5个参数的基本细胞分析。CompuCyte激光扫描细胞仪（LSC）提供与流式细胞术中类似的分子信号检测，不同之处在于样品固定在显微镜载玻片或细胞培养板上，而不是在流体悬浮液中。 这允许分析更多类型的组织和单个细胞的可视化。 Miltenyi Biotec autoMacs "细胞分选仪通过磁珠分离纯化亚群，用于进一步研究。 Luminex <$100 "分析可寻址激光珠阵列，用于捕获和检测小样品中的多种分析物。 Zeiss荧光显微镜和成像系统用于可视化和捕获荧光标记细胞的图像。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The function of the Fluorescence Cytometry Core Facility is to provide state-of-the art technology for investigators to aid them in their quest for understanding cellular processes involved in environmental health challenges. Understanding the processes at this level enables researchers to formulate and test theories as to how environmental factors affect health. These discoveries are important in the quest for disease prevention and treatment. The Core is home to instruments that use fluorescent markers and/or monoclonal antibodies to provide data that illuminate cellular processes. The BD FACSAria" is a state-of-the art flow cytometer and high-speed cell sorter. The three laser, nine filter-set configuration allows the identification of multiple molecular signals to be analyzed simultaneously. Further, the instrument has capabilities of precise sorting of subpopulations of interest for further investigation. The BD FACSCalibur" is a 1-laser, 3-detector flow cytometer that provides basic cell analysis in 5 parameters. The CompuCyte Laser Scanning Cytometer (LSC) offers similar detection of molecular signals as in flow Cytometry, the difference being that the sample is fixed on a microscope slide or cell culture plates, rather than in a fluid suspension. This allows more types of tissues to be analyzed and visualization of individual cells. The Miltenyi Biotec autoMacs" cell sorter purifies subpopulations by magnetic bead separation for further investigation. The Luminex¿ 100" analyzes addressable laser bead arrays for capture and detection of multiple analytes in small samples. The Zeiss" Fluorescence Microscope and Imaging System is used to visualize and capture images of fluorescently tagged cells.