FLOW CYTOMETRY ANALYSIS/ HIGH SPEED CELL SORTING

流式细胞术分析/高速细胞分选

• 批准号: 8360463
• 负责人: KEVAN ROBERTS
• 金额: $ 13.7万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360463
• 关键词: Affect; Cell Separation; Cell physiology; Cells; Core Facility; Cytometry; Environmental Health; Environmental Risk Factor; Flow Cytometry; Fluorescence; Funding; Grant; Health; Lasers; National Center for Research Resources; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resources; Scanning; Source; Speed; System; Technology; Testing; United States National Institutes of Health; cost; disorder prevention; experience; fluorescence imaging; fluorescence microscope; instrument; instrumentation; theories

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The function of the Fluorescence Cytometry Core Facility is to provide state-of-the art technology for investigators to aid them in their quest for understanding cellular processes involved in environmental health challenges. Understanding the processes at this level enables researchers to formulate and test theories as to how environmental factors affect health. These discoveries are important in the quest for disease prevention and treatment. The core provides experience and instrumentation in the field of flow cytometry and quantitative immuno-fluorescence imaging. The instruments present in the core include: 1. BD FACSAria Flow Cytometer and Cell Sorter 2. BD FACSCalibur Flow Cytometer 3. CompuCyte iCys Laser Scanning Cytometer 4. Luminex System (multi-analyte analyzer) 5. Miltenyi Biotec AutoMACS 6. Zeiss Fluorescence Microscope.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 荧光细胞计数核心设施的功能是为研究人员提供最先进的技术，以帮助他们了解环境健康挑战所涉及的细胞过程。 了解这一层次的过程使研究人员能够制定和测试理论，以环境因素如何影响健康。 这些发现对于疾病预防和治疗的探索非常重要。 该核心提供流式细胞术和定量免疫荧光成像领域的经验和仪器。核心文件中的文书包括： 1. BD FACSAria流式细胞仪和细胞分选仪 2. BD FACSCalibur流式细胞仪 3. CompuCyte iCys激光扫描细胞仪 4. Luminex系统（多分析物分析仪） 5. Miltenyi Biotec AutoMACS 6. 荧光显微镜.