COBRE: LSU: MOLECUL NEUROBIOL CORE RESOURCE MODULE: TRANSGENIC KNOCKOUT ANIMALS

COBRE：LSU：分子神经生物学核心资源模块：转基因敲除动物

• 批准号: 7959411
• 负责人: Nicolas G. Bazan
• 金额: $ 13.86万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959411
• 关键词: Animals; Caring; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; DNA Microarray Chip; Detection; Fluorescence; Funding; Gel; Gene Chips; Genotype; Grant; Housing; Image; Institution; Knock-out; Louisiana; Mentors; Methods; Molecular Neurobiology; Neurosciences; Postdoctoral Fellow; RNA; Research; Research Personnel; Resource Sharing; Resources; Sampling; Screening procedure; Services; Source; System; Technology; Tissues; Transgenic Organisms; United States National Institutes of Health; knockout animal; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Neurobiology Core Module (MNCM) is directed by Dr. Rene Anand and Dr. Erickson. Two Research Associates provide the services of the MNCM. The MNCM allows investigators to obtain high-quality images produced by fluorescence or chemiluminescence detection methods of samples including gels, blots, and microplates, using the Fluor-S MAX MultiImager System from BioRAD. This facility houses a Bioanalyzer Agilent Technologies RNA purity scanner. The facility provides guidance about extracting and purifying RNA from cells and tissues. This core is also a linkage with the gene chip analysis core that houses the Affymetrix 428 Array Scanner and 417 Arrayer for screening DNA microarrays. The MNCM also provides a high-quality shared resource for genotyping and other specialized care needed for maintaining stocks of transgenic and knock-out animals used during the course of the COBRE proposal.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 分子神经生物学核心模块（MNCM）由Rene Anand博士和Erickson博士指导。 两名研究助理提供MNCM的服务。 MNCM允许研究人员使用BioRAD的Fluor-S MAX MultiImager系统获得通过样品的荧光或化学发光检测方法产生的高质量图像，包括凝胶，印迹和微孔板。 该设施设有生物分析仪Agilent Technologies RNA纯度扫描仪。该设施提供了从细胞和组织中提取和纯化RNA的指导。该核心也是与基因芯片分析核心的连接，该核心容纳了用于筛选DNA微阵列的Affyoung 428阵列扫描仪和417阵列仪。 MNCM还提供了一个高质量的共享资源，用于基因分型和维持COBRE提案过程中使用的转基因和敲除动物种群所需的其他专业护理。