Center for the Analysis of Cellular Mechanisms and Systems Biology

细胞机制和系统生物学分析中心

• 批准号: 7692996
• 负责人: EDWARD A DRATZ
• 金额: $ 223.95万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7692996
• 关键词: Center; Analysis; Cellular; Mechanisms; Systems

Description (provided by applicant): The goal of this application is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center will be unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add: a high resolution Qtof MS for improved "bottom up" characterization of protein post-translational modifications, an FTMS for "top-down" characterization of modifications on intact proteins, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications that result from biological stimulation. We will build critical mass by first supporting the development of three young faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Second, we will hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced FTMS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support ($1,400,000), the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.

描述（由申请人提供）：本申请的目标是建立一个多学科的细胞机制和系统生物学分析中心，提供新的基础设施，以促进对细胞机制的理解，更好地了解如何克服疾病，并在该领域建立一个关键的师资队伍。该中心在蒙大拿州和周边五个州将是独一无二的，并将包括细胞机制分析的综合能力。该项目将创建一个强大的质谱和蛋白质组学核心基础设施，面向培训研究人员。大多数细胞机制似乎是由蛋白质翻译后修饰控制的，MS和Proteomics Core将补充：一个高分辨率Qtof质谱，用于改进蛋白质翻译后修饰的“自下而上”表征；一个FTMS，用于完整蛋白质修饰的“自上而下”表征；一个高光谱成像仪，用于增强多色蛋白质组学多路复用，以精确定位随生物刺激变化的蛋白质，以及一个蛋白质电洗脱系统，用于高效回收精确定位的蛋白质。敏感的微分