Center for the Analysis of Cellular Mechanisms and Systems Biology

该应用程序的目标是开发一个多学科的细胞机制和系统生物学分析中心，提供新的基础设施来促进对细胞机制的理解，更好地了解如何克服疾病，并在这一领域建立一支关键的教师队伍。该中心将在蒙大拿州和周围的五个州独一无二，并将包括分析细胞机制的能力组合。该项目将创建一个强大的质谱学和蛋白质组学核心基础设施，旨在培训研究人员。大多数细胞机制似乎受蛋白质翻译后修饰的控制，MS和蛋白质组学核心将增加：高分辨率QtOf MS用于改进蛋白质翻译后修饰的“自下而上”表征，FTMS用于完整蛋白质修饰的“自上而下”表征，高光谱成像仪用于增强多色蛋白质组多路传输到随生物刺激变化的针点蛋白质，以及蛋白质电洗脱系统用于高效回收针尖蛋白质。核心将包括密歇根州立大学开发的灵敏差异蛋白质检测能力和高灵敏微流控纳米LC/MS/MS。新的软件将整合来自现有DNA微阵列设施的数据。蛋白质组学试剂合成核心将制备新的荧光试剂，用于差异分析生物刺激导致的酶活性变化和翻译后修饰。 我们将通过首先支持三名年轻教员和一名资深教员的发展来建立临界质量，这名教员正在制定他的研究的新路线。优秀的地方和国家导师和外部咨询委员会成员将指导这些教师和中心的发展。其次，我们将在需要建立临界质量的领域招聘四名新教师：一名化学生物学家/有机合成专家，一名使用先进FTMS工具的生物质谱学家，一名从事代谢建模和代谢组学工作的系统生物学家，以及一名使用全球分子工具的细胞生物学家。杰出的密歇根州立大学机构支持(140万美元)，细胞机制的后基因组分析的重要性和及时性，以及对先进方法培训的导向，将催生新的项目--并将产生重大的科学贡献和一个可持续的中心。

The goal of this application is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center will be unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add: a high resolution Qtof MS for improved "bottom up" characterization of protein post-translational modifications, an FTMS for "top-down" characterization of modifications on intact proteins, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications, that result from biological stimulation. We will build critical mass by first supporting the development of three young faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Second, we will hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced FTMS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support ($1,400,000), the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.